Detection of Indicator Organisms from Potable Jar Water Samples in Dhaka City, Bangladesh

Aim: This present study was conducted to determine the presence of indicator microorganisms from potable jar water samples. Methods: A total of 30 jar water samples were collected from different areas in Dhaka city, Bangladesh. Samples were analyzed using conventional standard microbiological method such as membrane filtration technique and biochemical tests and VITEK 2 method for presumptive identification of the bacterial isolates. The outcomes from both methods were compared. Results: The high number of coliform and fecal coliform were found in Manda and Siddheswari area which were 225 CFU/100 ml and 166 CFU/100 ml respectively. The antibiotic susceptibility patterns of these isolates were further confirmed by Kirby Bauer disk diffusion method and automated system such as VITEK-2 compact. Antibiotic susceptibility testing showed that indicator organisms such as Escherichia coli and Klebsiella pneumoniae were 100% resistant to Cefuroxime, Vancomycin and Cefepime. Conclusions: This study indicated that some water samples were polluted by both coliform and fecal coliform which meant those potable jar water samples were of poor quality that may cause water borne diseases in consumers. The constant surveillance for drug resistance and microbiological analysis is crucial for efficient water borne disease prevention and treatment.