IDEAS home Printed from https://ideas.repec.org/a/bjc/journl/v12y2025i3p635-646.html
   My bibliography  Save this article

Genetic Profiling and Prevalence of Kelch 13 Propeller Gene Mutations in Plasmodium Falciparum Isolates from Nasarawa North Senatorial District, Nigeria

Author

Listed:
  • Timshana Emmanuel

    (Department of Microbiology, Nasarawa State University, PMB 1022, Keffi, Nasarawa State)

  • Ngwai Yakubu Boyi

    (Department of Microbiology, Nasarawa State University, PMB 1022, Keffi, Nasarawa State)

  • Igbawua Isaac Nyiayem

    (Department of Microbiology, Nasarawa State University, PMB 1022, Keffi, Nasarawa State)

Abstract

Malaria continues to pose a significant public health challenge in Nasarawa North Senatorial District, Nigeria, with increasing concerns about drug resistance. This study investigated the prevalence and genetic diversity of Plasmodium falciparum by analyzing Kelch 13 propeller gene mutations, a key marker for artemisinin resistance. A cross-sectional study was conducted across selected healthcare facilities, where 384 venous blood samples were collected and analyzed using microscopy, rapid diagnostic test kits (RDTs), and polymerase chain reaction (PCR). A total of 107 P. falciparum-positive cases were identified, and 27 samples were successfully sequenced. The results revealed a P. falciparum prevalence of 27.9%, with a mutation prevalence of 11.11%, with three-point mutations (D57V, A158C, and A157G) detected from P. falciparum isolates. General Hospital Akwanga recorded the highest mutation frequency (7.40%), while no Kelch 13 mutations were detected at General Hospital Nasarawa Eggon and Wamba. Statistical analysis (χ² = 8.02, p = 0.046) showed a significant variation in mutation distribution across healthcare facilities, pointing to area-specific factors influencing mutation prevalence. These findings emphasize the importance of continuous genetic surveillance to monitor resistance trends and guide malaria control strategies. Further research should explore the functional implications of Kelch 13 mutations to assess their potential impact on artemisinin efficacy.

Suggested Citation

  • Timshana Emmanuel & Ngwai Yakubu Boyi & Igbawua Isaac Nyiayem, 2025. "Genetic Profiling and Prevalence of Kelch 13 Propeller Gene Mutations in Plasmodium Falciparum Isolates from Nasarawa North Senatorial District, Nigeria," International Journal of Research and Scientific Innovation, International Journal of Research and Scientific Innovation (IJRSI), vol. 12(3), pages 635-646, March.
    • Handle: RePEc:bjc:journl:v:12:y:2025:i:3:p:635-646
    as

    Download full text from publisher

    File URL: https://www.rsisinternational.org/journals/ijrsi/digital-library/volume-12-issue-3/635-646.pdf
    Download Restriction: no
    File URL: https://rsisinternational.org/journals/ijrsi/articles/genetic-profiling-and-prevalence-of-kelch-13-propeller-gene-mutations-in-plasmodium-falciparum-isolates-from-nasarawa-north-senatorial-district-nigeria/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Frédéric Ariey & Benoit Witkowski & Chanaki Amaratunga & Johann Beghain & Anne-Claire Langlois & Nimol Khim & Saorin Kim & Valentine Duru & Christiane Bouchier & Laurence Ma & Pharath Lim & Rithea Lea, 2014. "A molecular marker of artemisinin-resistant Plasmodium falciparum malaria," Nature, Nature, vol. 505(7481), pages 50-55, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Krittikorn Kümpornsin & Theerarat Kochakarn & Tomas Yeo & John Okombo & Madeline R. Luth & Johanna Hoshizaki & Mukul Rawat & Richard D. Pearson & Kyra A. Schindler & Sachel Mok & Heekuk Park & Anne-Ca, 2023. "Generation of a mutator parasite to drive resistome discovery in Plasmodium falciparum," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Shiroh Iwanaga & Rie Kubota & Tsubasa Nishi & Sumalee Kamchonwongpaisan & Somdet Srichairatanakool & Naoaki Shinzawa & Din Syafruddin & Masao Yuda & Chairat Uthaipibull, 2022. "Genome-wide functional screening of drug-resistance genes in Plasmodium falciparum," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Amy Ibrahim & Franziska Mohring & Emilia Manko & Donelly A. Schalkwyk & Jody E. Phelan & Debbie Nolder & Steffen Borrmann & Ayola A. Adegnika & Silvia Maria Santi & Mohammad Shafiul Alam & Dinesh Mond, 2024. "Genome sequencing of Plasmodium malariae identifies continental segregation and mutations associated with reduced pyrimethamine susceptibility," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    4. Madhvi Chahar & Anup Anvikar & Neena Valecha, 2019. "Development and Evaluation of a Novel HNB Based Isothermal Amplification Assay for Fast Detection of Pyrimethamine Resistance (S108N) in Plasmodium falciparum," IJERPH, MDPI, vol. 16(9), pages 1-13, May.
    5. Tran Dang Nguyen & Bo Gao & Chanaki Amaratunga & Mehul Dhorda & Thu Nguyen-Anh Tran & Nicholas J. White & Arjen M. Dondorp & Maciej F. Boni & Ricardo Aguas, 2023. "Preventing antimalarial drug resistance with triple artemisinin-based combination therapies," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    6. Eric Zhewen Li & Tran Dang Nguyen & Thu Nguyen-Anh Tran & Robert J. Zupko & Maciej F. Boni, 2024. "Assessing emergence risk of double-resistant and triple-resistant genotypes of Plasmodium falciparum," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    7. Mariateresa Cesare & Mulenga Mwenda & Anna E. Jeffreys & Jacob Chirwa & Chris Drakeley & Kammerle Schneider & Brenda Mambwe & Karolina Glanz & Christina Ntalla & Manuela Carrasquilla & Silvia Portugal, 2024. "Flexible and cost-effective genomic surveillance of P. falciparum malaria with targeted nanopore sequencing," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    8. Patrick K. Tumwebaze & Melissa D. Conrad & Martin Okitwi & Stephen Orena & Oswald Byaruhanga & Thomas Katairo & Jennifer Legac & Shreeya Garg & David Giesbrecht & Sawyer R. Smith & Frida G. Ceja & Sam, 2022. "Decreased susceptibility of Plasmodium falciparum to both dihydroartemisinin and lumefantrine in northern Uganda," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:bjc:journl:v:12:y:2025:i:3:p:635-646. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Dr. Renu Malsaria (email available below). General contact details of provider: https://rsisinternational.org/journals/ijrsi/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.