“Molecular Detection and Clinical Characterization of Human Respiratory Adenovirus in Pakistani Childrenâ€

Background: A study in Pakistan examined HAdV prevalence in pediatric patients with severe respiratory illness. It correlated HAdV infection with CBC and X-ray abnormalities. CBC abnormalities included WBC, neutrophil, and lymphocyte counts. X-rays revealed pulmonary abnormalities like infiltrates and consolidation. Integrating CBC and X-ray findings improves diagnostic and management strategies for HAdV infections in pediatric patients, aiding in severe respiratory illness contexts. Methods: A cross-sectional study was carried out at the Children Health Care Hospital Balambat Temargara Dir Lower involving 389 children under five years old. Respiratory swabs were collected from 14 hospitals across Pakistan from October 22, 2023, and March 31, 2024, Demographics, signs, and symptoms were recorded using a proferma. Real-time polymerase chain reaction (RT-PCR) was used for respiratory samples to confirm HAdV. Following confirmation, blood samples were collected in EDTA tubes, and chest X-rays were performed for eachparticipant. Results: Out of 389 samples, our study reveals a gender disparity, with 58.7% male and 41.3% female patients, prompting investigation into healthcare-seeking behaviors. Additionally, all cases (100%) exhibited bilateral pneumonic infiltrates, highlighting the severity of Human Adenovirus infections in children. This underscores the need for timely diagnosis and intervention to improve outcomes, especially in pediatric populations. Conclusion: The findings of this study highlight a concerning trend: HAdV infection appears to be prevalent in Pakistan, particularly among female patients aged 1–6 months. This underscores the urgent need to enhance our diagnostic capabilities for detecting HAdV infections within our healthcare system. By doing so, we can effectively mitigate the risk of complications linked to this virus, ultimately safeguarding the health and well-being of our population, especially vulnerable groups like infants. Additionally, conducting genetic analyses could provide valuable insights into the specific genotypes of HAdV circulating in Pakistan. Such information would not only aid in refining diagnostic methods but also in developing targeted strategies for prevention and treatment tailored to the unique genetic characteristics of the virus strains present in our region.