Physico-Chemical Analysis and Phytochemical Characterization of Catharanthus Roseus (L.) G. Don in Contaminated Soil and Garden Soil

The physico-chemical characteristics and phytochemical profiles of Catharanthus roseus (L.) G. Don grown in both garden soil and contaminated soil are examined in this study. The purpose of the study is to evaluate how soil pollution affects that plant’s growth characteristics, metabolic components, and secondary metabolites. While plant samples were assessed for growth metrics, and phytochemical substances like alkaloids, flavonoids, and phenolics, soil samples were examined for pH, Dissolved solids, Dissolved oxygen and Total hardness and Calcium hardness. The Qualitative test for plant extract of solvent methanol and distilled water is done by Harbone’s Qualitative method for detection of secondary metabolites i.e., Alkaloid, Tannin, Phenol, Flavonoid, Saponins and Lignin. The determination of the Total Phenolic Content, Total Tannin Content and Total Flavonoid Content was done by Quantitative analysis with the help of Folin- ciocalteu, Folin- Phenol and Aluminium Chloride methods respectively. Determination of Total Antioxidant Capacity id done by Phosphomolybdenum Assay. The test results of quantitative are all using the concentration of 1 ml of prepare sample extract. The plant extract of solvent methanol and distilled water detects many secondary metabolites like Alkaloid, tannin, phenol, flavonoid, saponins and lignin this all are detect in the phytochemical test by qualitative methods and according to quantitative analysis the pollution affects the phytochemical but the Antioxidant capacity remains same. The findings demonstrate the adaptability of Catharanthus roseus (L.) G. Don under challenging circumstances and its potential for application in phytoremediation by showing notable differences in growth and phytochemical composition between plants in contaminated areas and their consequences for environmental health and sustainable agriculture are better understood thanks to this research.