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First Detection of Shv-Type Extended Spectrum Β-Lactamases in The University Hospital Complex Paediatric Charles De Gaulle (CHUP-CDG) of Ouagadougou in Burkina Faso

Author

Listed:
  • Mètuor Dabiré Amana
  • Zongo K Jacob
  • Zeba Boukaré
  • Traoré/Ouedraogo Rasmata
  • Moussawi Jihad
  • Baucher Marie
  • El Jaziri Mondher

Abstract

Strains were isolated from urines clinical samples from patients from various service units of University Hospital complex Paediatric Charles De Gaulle (CHUP-CDG) in Burkina Faso. These strains were resistant to at least one oxyimino-cephalosporin. They were identified as producer of extended spectrum β-lactamases (ESBL) by double-disk synergy test between amoxicillin–clavulanate and cefotaxime, ceftriaxone or ceftazidime and by kinetic methods. Crude extract from these isolates showed a broad-substrate profile by hydrolyzing cefuroxime, cefotaxime, ceftriaxone and with a lower degree to cetazidime and cefepime but not imipinem. Sequencing of PCR products amplified with primers designed for blaSHV genes showed that these genes coded for SHV-11, SHV-12, SHV-28, SHV-32, SHV-38, SHV-76 and SHV-99. This is the first description of these enzymes in Burkina Faso.

Suggested Citation

  • Mètuor Dabiré Amana & Zongo K Jacob & Zeba Boukaré & Traoré/Ouedraogo Rasmata & Moussawi Jihad & Baucher Marie & El Jaziri Mondher, 2014. "First Detection of Shv-Type Extended Spectrum Β-Lactamases in The University Hospital Complex Paediatric Charles De Gaulle (CHUP-CDG) of Ouagadougou in Burkina Faso," Journal of Asian Scientific Research, Asian Economic and Social Society, vol. 4(5), pages 214-221.
  • Handle: RePEc:asi:joasrj:v:4:y:2014:i:5:p:214-221:id:3629
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