IDEAS home Printed from https://ideas.repec.org/a/arp/rjbarp/2018p80-82.html
   My bibliography  Save this article

Construction, Expression, and Purification of ZNF191 (243-368) Zinc Finger Deletion Mutants

Author

Listed:
  • Dongxin Zhao*

    (College of Chemistry, Chemical and Environmental Engineering, Henan University of Technology, Zhengzhou, Henan, China)

  • Zhongxian Huang

    (Department of Chemistry, Fudan University, Shanghai, China)

Abstract

The C-terminal region of the zinc finger protein ZNF191(243-368) contains a putative DNA-binding domain containing four Cys2His2 zinc finger motifs. To understand the properties and functions of the zinc finger motifs, a deletion gene of ZNF191(243-368) was inserted into pGEX-4T-2. The recombinant vector was transformed into Escherichia coli BL21, and a glutathione S-transferase (GST) fusion protein was expressed and purified using glutathione agarose affinity resin. The results show that this expression system can be used to express and purify zinc finger deletion mutants of ZNF191(243-368), providing a basis for further investigation of this protein.

Suggested Citation

  • Dongxin Zhao* & Zhongxian Huang, 2018. "Construction, Expression, and Purification of ZNF191 (243-368) Zinc Finger Deletion Mutants," Journal of Biotechnology Research, Academic Research Publishing Group, vol. 4(11), pages 80-82, 11-2018.
    • Handle: RePEc:arp:rjbarp:2018:p:80-82
    DOI: 10.32861/jbr.411.80.82
    as

    Download full text from publisher

    File URL: https://www.arpgweb.com/pdf-files/jbr4(11)80-82.pdf
    Download Restriction: no
    File URL: https://www.arpgweb.com/journal/16/archive/11-2018/11/4
    Download Restriction: no
    File URL: https://libkey.io/10.32861/jbr.411.80.82?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:arp:rjbarp:2018:p:80-82. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Managing Editor (email available below). General contact details of provider: http://arpgweb.com/index.php?ic=journal&journal=16&info=aims .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.