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Evaluation of Extraction Methods of DNA from Dry Collection Material of Urophora cuspidata and Urophora macrura (Diptera: Tephritidae)

Author

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  • Fahriye Sumer Ercan

    (Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Ahi Evran University, 40200, Kırşehir, Turkey)

  • Neslihan Bayrak

    (Department of Plant Protection, Faculty of Agriculture and Natural Sciences, Bozok University, 66900, Yozgat, Turkey)

  • Sevim Dogan

    (Department of Plant Protection, Faculty of Agriculture and Natural Sciences, Bozok University, 66900, Yozgat, Turkey)

Abstract

Different DNA extraction protocols are evaluated for DNA isolation from various samples. In our study we compared three DNA extraction methods; a Chelex resin (C100), Qiagen DNA extraction kit and Cethyl Trimethyl Ammonium Bromide (CTAB) protocols obtained from dry collected materials of Urophora cuspidata and Urophora macrura (Diptera: Tephritidae) samples. Although, the hihgest yield of DNA was obtained from C100 method, the purest DNA was obtained with Qiagen protocol. Using RAPD-PCR, we demonstrate the efficacy of Qiagen protocol on these samples collected up to 6 years ago.Â

Suggested Citation

  • Fahriye Sumer Ercan & Neslihan Bayrak & Sevim Dogan, 2016. "Evaluation of Extraction Methods of DNA from Dry Collection Material of Urophora cuspidata and Urophora macrura (Diptera: Tephritidae)," Journal of Biotechnology Research, Academic Research Publishing Group, vol. 2(1), pages 1-5, 01-2016.
    • Handle: RePEc:arp:rjbarp:2016:p:1-5
    DOI: arpgweb.com/?ic=journal&journal=16&info=aims
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