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The Application of Modern Biotechnology in Protein Interaction Research-A Review

Author

Listed:
  • Bo Peng

    (College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China)

  • Xia-Yu Tian

    (College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China)

  • Zi-Yue Liu Author-Workplace-Name College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China:
  • Juan Peng Author-Workplace-Name Xinyang Station of Plant Protection and Inspection, Xinyang, China
  • Meng-Yang Zheng

    (College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China)

  • Xiao-Hua Song

    (Xinyang Academy of Agricultural Science, Xinyang, China)

  • Ya-Qin Huang

    (College of Biological and Pharmaceutical Engineering, Xinyang Agricultural and Forestry University, Xinyang, China)

  • Xin-Xiang A

    (Yunnan Provincial Key Lab of Agricultural Biotechnology of Yunnan Academy of Agricultural Sciences/ Scientific Observation Station for Rice Resource of Yunnan, Ministry of Agriculture, Kunming, China)

  • Yan-Ming Zhang

    (State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China)

  • Fang Yang Author-Workplace-Name State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China:
  • Hui-Long Li

    (Xinyang Academy of Agricultural Science, Xinyang, China)

  • Yan-Yang Sun

    (College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China)

  • Rui-Hua Pang

    (College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China)

  • Wei Zhou

    (College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China)

  • Quan-Xiu Wang

    (College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China)

  • Hongyu Yuan

    (College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China)

Abstract

Intermolecular interaction is the material basis for cells to achieve their functions, and protein-protein interaction is an important approach to illuminate the regulation network of biological molecules and has important theoretical significance and potential application value for revealing the activity law of life in nature. This paper mainly summarizes and analyzes the new advances and applications of modern biotechnologies in the study of protein-protein interactions, including local surface plasmon resonance (LSPR), yeast two-hybrid, GST-Pull-down, bimolecular fluorescence complementation, and co­immunoprecipitation. At the same time, the principles of different research methods for protein-protein interaction and their other applications in the field of life sciences are also discussed, all of these will provide a reference value for the analysis of protein-protein interaction and the molecular regulation mechanism of biomacromolecules.

Suggested Citation

  • Bo Peng & Xia-Yu Tian & Zi-Yue Liu Author-Workplace-Name College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xin, 2021. "The Application of Modern Biotechnology in Protein Interaction Research-A Review," Journal of Agriculture and Crops, Academic Research Publishing Group, vol. 7(2), pages 39-47, 04-2021.
    • Handle: RePEc:arp:jacarp:2021:p:39-47
    DOI: 10.32861/jac.72.39.47
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