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Characterization and Starch Properties of a Waxy Mutant in Japonica Rice Kitaake

Author

Listed:
  • Long Zhang

    (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province / Joint International Research Laboratory ofAgriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China)

  • Linglong Zhao

    (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province / Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China)

  • Xiurui Zhang

    (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province / Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China)

  • Lingshang Lin

    (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province / Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China)

  • Cunxu Wei*

    (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province / Joint International Research Laboratory of Agriculture & Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China)

Abstract

A rice waxy mutant M6 was generated from a japonica rice cultivar Kitaake through gamma irradiation. In this study, we characterized the mutant and analyzed its starch properties. The M6 with milky opaque kernels had lower seed length, width, and weight than wild type. The cavity in the center of starch granule might be responsible for waxy appearance of M6 mature kernels. Sequence analysis of granule-bound starch synthase I (GBSSI) gene showed that there was a 23 bp duplication inserted into the exon 2, generating one stop codon. No GBSSI protein was detected in the endosperm of M6. The isolated starch showed similar ratio of short and long branch-chains of amylopectin between M6 and wild type, but the M6 starch had no amylose. Both the M6 and wild type had A-type starch, but the M6 starch exhibited higher relative crystallinity than wild type starch. Compared with wild type starch, the M6 starch had significantly high swelling power, gelatinization enthalpy and breakdown viscosity and low water solubility, gelatinization peak temperature, peak viscosity, hot viscosity, final viscosity and setback viscosity. The M6 starch had significantly lower resistance to amylase hydrolysis than wild type starch.

Suggested Citation

  • Long Zhang & Linglong Zhao & Xiurui Zhang & Lingshang Lin & Cunxu Wei*, 2018. "Characterization and Starch Properties of a Waxy Mutant in Japonica Rice Kitaake," Journal of Agriculture and Crops, Academic Research Publishing Group, vol. 4(11), pages 117-124, 11-2018.
    • Handle: RePEc:arp:jacarp:2018:p:117-124
    DOI: 10.32861/jac.411.117.124
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