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Development of a Transient Viral CRISPR Expression System to Manipulate Flowering Time in Plants

Author

Listed:
  • CITRA RECHA SARI

    (School of Life Sciences, University of Warwick, CV4 7AL, United Kingdom)

  • JEMMA TAYLOR

    (School of Life Sciences, University of Warwick, CV4 7AL, United Kingdom)

  • YIGUO HONG

    (School of Life Sciences, University of Warwick, CV4 7AL, United Kingdom)

  • STEPHEN JACKSON

    (School of Life Sciences, University of Warwick, CV4 7AL, United Kingdom)

Abstract

Around 180 genes have been found in Arabidopsis that control flowering time based on analysis of transgenic plants [1]. Most of these genes take part in the six main pathways controlling flowering; the photoperiod, vernalization and the ambient temperature pathways that respond to environmental conditions, while the age-dependent, autonomous, and gibberellin pathways involve a response to endogenous signals [2]. Flowering Locus T (FT) is a major gene involved in flowering in plants because FT encodes mobile florigen for floral induction. Mutation of the FT gene can result in delayed flowering. Using a transient CRISPR-Cas9 expression system mediated by virus expression, it is possible to do gene-editing in plants without incorporation of exogenous DNA. We aim to develop these approaches to manipulate flowering time in plants

Suggested Citation

  • Citra Recha Sari & Jemma Taylor & Yiguo Hong & Stephen Jackson, 2016. "Development of a Transient Viral CRISPR Expression System to Manipulate Flowering Time in Plants," International Journal of Applied and Physical Sciences, Dr K.Vivehananthan, vol. 2(3), pages 65-70.
    • Handle: RePEc:apa:ijapss:2016:p:65-70
    DOI: 10.20469/ijaps.2.50002-3.pdf
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    Cited by:

    1. A. T. Mohammad & T. H. Darma & A. Barde & S. Isyaku & A. H. Umar, 2017. "Investigation of the morphology and optical properteis of CuAlO2 thin film," Journal of Applied and Physical Sciences, Prof. Vakhrushev Alexander, vol. 3(1), pages 17-25.
    2. Huynh Thi Thuy Hanh & Wiyada Mongkolthanaruk, 2017. "Correlation of growth and IAA production of Lysinibacillus Fusiformis UD 270," Journal of Applied and Physical Sciences, Prof. Vakhrushev Alexander, vol. 3(3), pages 98-106.

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