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Occurrence Of Two Branched Penta-Amines In Legume Seeds Has Not Been Confirmed

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  • Koei Hamana
  • Masaru Niitsu

Abstract

We detected N 4 -bis(aminopropyl)spermidine [3(3)(3)4], a quaternary branched penta-amine and N 5 -aminobutylhomospermine [4(4)44], a tertiary branched penta-amine by liquid chromatography and gas chromatography of the concentrated/purified polyamine fraction acid-extracted from the legume seeds of Crotalaria spectabilis, and other legume seeds of Vicia sativa (narrow-leaved vetch), Vicia villosa (hairy vetch) and Phaseolus coccineus (runner bean), respectively, and we have reported tentatively the natural occurrence of the two penta-amines in legume seeds. We validated and evaluated the analyses of the two penta-amines in our previous studies and showed here some unreproducible polyamine profiles among the seeds supplied from markets, which we cultivated and collected in fields. Contamination of the branched penta-amines during concentration/purification to obtain polyamine fraction before performing the two chromatographical analyses, was not excluded. Therefore, the natural occurrence of the two branched penta-amines in leguminous plant seeds has not been confirmed.

Suggested Citation

  • Koei Hamana & Masaru Niitsu, 2025. "Occurrence Of Two Branched Penta-Amines In Legume Seeds Has Not Been Confirmed," International Journal of Agriculture and Environmental Research, Malwa International Journals Publication, vol. 11(05), October.
    • Handle: RePEc:ags:ijaeri:376234
    DOI: 10.22004/ag.econ.376234
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