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Culturing and population studies of Ditylenchus dipsaci under monoxenic conditions

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  • Viglierchio, D. R.
  • Siddiqui, I. A.
  • Croll, N. A.

Abstract

Ditylenchus dipsaci is well known for its “race” characteristics in terms of host range preferences. This study established isolates of D. dipsaci occurring in California in monoxenic laboratory cultures to account for different host properties as reflected in nematode growth and development. Microbial contaminants in hosts as well as seed lots varied, thus no single axenization method was found acceptable. Multiple treatments, varying in kinds and sequences of axenizing agents and duration of exposures, were required. Growth rate of all tissues were increased two-fold by the addition of casein hydrolysate and yeast hydrolysate to a literature recommended medium.

Suggested Citation

  • Viglierchio, D. R. & Siddiqui, I. A. & Croll, N. A., 1973. "Culturing and population studies of Ditylenchus dipsaci under monoxenic conditions," Hilgardia, California Agricultural Experiment Station, vol. 42(6).
    • Handle: RePEc:ags:hilgar:381825
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