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Key Technology for Virus-free Seed Potatoes Pre-elite Breeding

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  • XU, Jie
  • SUN, Li
  • ZHU, Wenting

Abstract

[Objectives] To establish a breeding system for pre-elite seed potatoes. [Methods] Shoot-tip seedlings were cultured with different medium, and then transplanted in different substrates. They were sprayed with nutrient solutions of different formulas. Potato minitubers were induced. [Results] The medium of MS+2.0 mg/L 6-BA, 1.0 mg/L NAA+3% sugar was preferable for potato shoot tip culture. After virus detection, the virus-free potato seedlings were propagated. The medium composed of vermiculite, peat and perlite (1:3:1) was recommended for transplanting potato tissue culture seedlings in greenhouse. The three-dimensional spray method was used to produce pre-elite seed potatoes. The full-amount MS nutrient solution could increase the tuber number per plant to 55. Induction of minitubers is a better breeding technology for pre-elite seed potatoes. The optimal medium screened for induction of potato minitubers was MS + (5-7) mg/L 6-BA, with a dark culture duration of about 20 d. [Conclusions] The breeding technology established is suitable for the two-season cultivation areas in ​​Zaozhuang.

Suggested Citation

  • XU, Jie & SUN, Li & ZHU, Wenting, 2022. "Key Technology for Virus-free Seed Potatoes Pre-elite Breeding," Asian Agricultural Research, USA-China Science and Culture Media Corporation, vol. 14(03), March.
    • Handle: RePEc:ags:asagre:330030
    DOI: 10.22004/ag.econ.330030
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    Agribusiness;

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