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Establishment and Optimization of Two-dimensional Electrophoresis System for Spleen Proteome of Sillago sihama Forsskål

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  • WANG, Junlin
  • CHEN, Xiulan
  • HU,Zhonghong
  • LI,Jing
  • PANG,Huanying
  • HUANG,Yang

Abstract

Taking Sillago sihama Forsskål as the research object, through the optimization of the extraction method, hydration and isoelectric focusing, a two-dimensional electrophoresis system was established for spleen proteome of S. sihama Forsskål. The results showed that the twp-step hydration and isoelectric focusing method is better than the hydration and isoelectric focusing integrated method. The protein spots of the spleen of S. sihama Forsskål were detected by two-dimensional electrophoresis mainly at pH 4.0-7.0. The establishment of this technical system will lay a foundation for further research on proteomics of S. sihama Forsskål in the future.

Suggested Citation

  • WANG, Junlin & CHEN, Xiulan & HU,Zhonghong & LI,Jing & PANG,Huanying & HUANG,Yang, 2019. "Establishment and Optimization of Two-dimensional Electrophoresis System for Spleen Proteome of Sillago sihama Forsskål," Asian Agricultural Research, USA-China Science and Culture Media Corporation, vol. 11(03), March.
    • Handle: RePEc:ags:asagre:290297
    DOI: 10.22004/ag.econ.290297
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    Agribusiness;

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