IDEAS home Printed from https://ideas.repec.org/a/adm/journl/v8y2019i3p88-93.html
   My bibliography  Save this article

Studying the Features of CRISPR Loci in Stenotrophomonas

Author

Listed:
  • Hengxia Fu
  • Mengmeng Zhang
  • Jiangnan Qin
  • Wei Luo
  • Bin Wang

Abstract

To study the genetic structure of the Stenotrophomonas CRISPR-Cas system using bioinformatics methods. Methods The sequence information of all Stenotrophomonas strains published in the CRISPRdb database was collected, and the CRISPR locus was analyzed using the CRISPRFinder software; All spacers were searched by BLAST platform in the PubMed database to find homologous sequences, and then the relationship between the number of spacer sequences and the number of phages was statistically analyzed. Results According to statistics, 15 confirmed CRISPR structures and 132 questionable CRISPRs were found in 26 strains of Stenotrophomonas, and the repeat sequences of CRISPR structures in different strains were more conservative. Only 1.3% of spacers were homologous with the sequences of known bacteriophages or plasmid in NCBI database. Conclusion The targeted genes of the spacer sequences are mainly from the genome of the bacteria, indicating that the evolution of the Stenotrophomonas CRISPR is related to other bacterial genes. In addition, the negative correlation between the spacer sequence and the number of phages indicates that CRISPR can prevent phage invasion. Analysis of the structure of CRISPR loci in the genome of Stenotrophomonas laid the foundation for further study of drug resistance and genomic stability.

Suggested Citation

  • Hengxia Fu & Mengmeng Zhang & Jiangnan Qin & Wei Luo & Bin Wang, 2019. "Studying the Features of CRISPR Loci in Stenotrophomonas," International Journal of Sciences, Office ijSciences, vol. 8(03), pages 88-93, March.
    • Handle: RePEc:adm:journl:v:8:y:2019:i:3:p:88-93
    DOI: 10.18483/ijSci.1978
    as

    Download full text from publisher

    File URL: https://www.ijsciences.com/pub/article/1978
    Download Restriction: no
    File URL: https://www.ijsciences.com/pub/pdf/V82019031978.pdf
    Download Restriction: no
    File URL: https://libkey.io/10.18483/ijSci.1978?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:adm:journl:v:8:y:2019:i:3:p:88-93. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Staff ijSciences (email available below). General contact details of provider: .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.