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Inhibitory Effect of Phytic Acid on LPS Induced BV-2 Microglia Activation

Author

Listed:
  • Jinhui Zuo
  • Fuping Guan
  • Xin Li
  • Xiuli Wang
  • Fengyu Pan
  • Zheng Zhang
  • Chao Song

Abstract

Hyperactivation of microglia is the important pathology of neurodegenerative diseases. Being naturally occurring compound, phytic acid (PA) has been found to exert anti-inflammatory effect in MPTP-induced parkinson’s disease model of mice, but mechanism needed to be further investigated. In our study, BV-2 microglia were cultured and induced by lipopolysaccharide (LPS) to mimic inflammation models to testify the phytic acid’s effect on neuroinflammation. Methods: BV-2 microglia were cultured in vitro. After pretreatment with different doses of PA, lipopolysaccharide (LPS) was added to induce the activation of BV-2 microglia. The cell survival rate was detected by MTT colorimetry, the NO release of microglia was measured by Griess assay, and the concentration of IL-6 in supernatant of cell culture was detected by ELISA assay. DAPI staining was used to detect the changes of apoptosis of activated BV-2 microglia before and after phytic acid treatment. Results: Phytic acid could inhibit the production of NO and IL-6 in BV-2 microglia activated by LPS, and block the excessive activation of BV-2 microglia stimulated by LPS. At the same time, it can promote the apoptosis of over-activated microglia. Therefore, phytic acid may play a potential role on prevention and treatment of neurodegenerative diseases associated with its inhibition on microglial activation.

Suggested Citation

  • Jinhui Zuo & Fuping Guan & Xin Li & Xiuli Wang & Fengyu Pan & Zheng Zhang & Chao Song, 2019. "Inhibitory Effect of Phytic Acid on LPS Induced BV-2 Microglia Activation," International Journal of Sciences, Office ijSciences, vol. 8(03), pages 8-11, March.
    • Handle: RePEc:adm:journl:v:8:y:2019:i:3:p:8-11
    DOI: 10.18483/ijSci.1901
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