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Rp-Hplc Method For Simultaneous Determination Of Metronidazole And Ofloxacin In Synthetic Mixture

Author

Listed:
  • Vania Maslarska

    (Medical University of Sofia)

  • Boyka Tsvetkova

    (Medical University of Sofia)

  • Lily Peikova

    (Medical University of Sofia)

  • Stanislav Bozhanov

    (Medical University of Sofia)

Abstract

Development and validation of a high-performance liquid chromatographic analytical procedure for simultaneously determining metronidazole and ofloxacin in a synthetic mixture is described in this paper. The separation was made with a LiChrosorb® RP-18 (250 × 4.6 mm) column, at 30 °C temperature, with isocratic mode and mobile phase, containing triethylamine, acetonitrile, and 0.3% o-phosphoric acid solution (0.02:20:80 v/v/v). Eluent was monitored at 290 nm and the flow rate was 1.0 ml/min. Metronidazole and ofloxacin were effectively separated with retention time (tr) of 3.42 min and 6.15 min, respectively, within the selected chromatographic conditions. The method was validated for analytical parameters: specificity, linearity, precision, accuracy, and limits of detection and quantitation. The calibration curves were linear in the concentration range of 12.5 to 100.0 µg/ml for metronidazole and ofloxacin, and the regression coefficients were more than 0.999. For metronidazole and ofloxacin the recovery was 100.01% and 100.04%, respectively. This analytical procedure is applicable for the quality control of drug formulations.

Suggested Citation

  • Vania Maslarska & Boyka Tsvetkova & Lily Peikova & Stanislav Bozhanov, 2016. "Rp-Hplc Method For Simultaneous Determination Of Metronidazole And Ofloxacin In Synthetic Mixture," CBU International Conference Proceedings, ISE Research Institute, vol. 4(0), pages 900-905, September.
    • Handle: RePEc:aad:iseicj:v:4:y:2016:i:0:p:900-905
    DOI: 10.12955/cbup.v4.871
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