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Fat Soluble Vitamins And Fatty Acids Composition Of Black Sea Cystoseira Barbata

Author

Listed:
  • Veselina Panayotova

    (Medical University of Varna)

  • Mona Stancheva

    (Medical University of Varna)

Abstract

Brown alga Cystoseira barbata is the most widely distributed seaweed in the Black Sea. There is limited information about fat soluble vitamins content and fatty acids composition of this specie from Bulgarian Black Sea coast. The aim of this study was to determine fat soluble vitamins, pigments, total lipid and fatty acid composition of Cystoseira barbata. Fat soluble vitamins (vitamin E and D), pigments (β-carotene and astaxanthin) and total cholesterol were analyzed simultaneously using HPLC/UV/FL system equipped with RP analytical column. Sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Brown seaweed Cystoseira barbata contained high amounts of α-tocopherol and β-carotene. Lipids were extracted by following the method of Bligh and Dyer. The residual lipid fraction was methylated using base-catalyzed transmethylation with methanolic potassium hydroxide. Fatty acid composition was analyzed by GC/MS. Cystoseira barbata was rich in linoleic (C18:2n6) and eicosopentaenoic acid (C20:5n3) although total lipid content was generally low. High levels of α-tocopherol correlate with high levels of polyunsaturated fatty acids. As an antioxidant α-tocopherol preserves tissue PUFA from oxidation.

Suggested Citation

  • Veselina Panayotova & Mona Stancheva, 2013. "Fat Soluble Vitamins And Fatty Acids Composition Of Black Sea Cystoseira Barbata," CBU International Conference Proceedings, ISE Research Institute, vol. 1(0), pages 362-367, June.
    • Handle: RePEc:aad:iseicj:v:1:y:2013:i:0:p:362-367
    DOI: 10.12955/cbup.v1.58
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