Effect of Various Oils on the Production of Lipase by Rhodotorula mucilaginosa and Yarrowia lipolytica Isolated from Spoiled Labneh

The content of the medium is very important in the production of lipase enzyme. Microbial lipase production is carried out by adding carbon source to the compounds in the minimal medium. The use of olive oil is quite common in lipase production medium. In this study, it was aimed to increase lipase production by adding different oils instead of olive oil to the reference lipase production medium. For this purpose, corn oil, sunflower seed oil, walnut oil, hazelnut oil, safflower oil, avocado oil, organic sunflower seed oil, organic canola seed oil, organic flaxseed oil, organic soybean oil, pumpkin seed oil, black cumin oil, St. John’s wort oil and sesame oil were evaluated in terms of lipase production. Rhodotorula mucilaginosa and Yarrowia lipolytica strains, which were isolated, purified, and identified, and which caused spoilage in labneh were studied in lipase production. When olive oil was used in lipase production, the lipase activity of R. mucilaginosa was determined as 7.50 U/ml, and that of Y. lipolytica was determined as 8.09 U/ml. It was determined that the lipase activity of R. mucilaginosa was increased by organic linseed oil as 10.67 U/ml, St. John’s Wort oil as 10.17 U/ml, and organic sunflower oil as 9.67 U/ml, respectively. It was determined that the lipase activity of Y. lipolytica was increased by St. John’s Wort oil as 13.50 U/ml, organic linseed oil as 10.84 U/ml, and organic sunflower oil as 10.33 U/ml, respectively. It was also observed that black cumin oil inhibited the growth of both yeast strains.