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An Overview of Aseptic Strategies Applied for In-house Polymerase Chain Reaction Techniques Discussed with Trouble Shootings

Author

Listed:
  • Kathiravan Vayanaperumal

    (Sankara Nethralaya Referral Lab, Medical Research Foundation, India)

  • Ranjith Kumar

    (Sankara Nethralaya Referral Lab, Medical Research Foundation, India)

Abstract

Polymerase chain reaction which is the widely utilized method had made progressive imprint in scientific research has its solid focuses and inadequacies. It presents the chance of defilement through the spreading of amplicon mist concentrates in the laboratory environment. To help lessen the probability of tainting, good laboratory practice ought to be practiced consistently. This review is centers to setting up a PCR laboratory and techniques for counteraction and devastation of contamination, and quality control.

Suggested Citation

Handle: RePEc:epw:ejbio0:v:1:y:2020:i:6:id:17119
DOI: 10.24018/ejbio.2020.1.6.119
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