Angiogenic Effects of Selected Food Seasonings on Fertilized Duck Embryo via the CAM Assay

Introduction: Angiogenesis is a vital physiological process involved in growth, development, and wound healing, but it may be adversely influenced by environmental and dietary factors. Despite the widespread consumption of food seasonings, the potential effects of their bioactive components—such as monosodium glutamate (MSG)—on angiogenesis remain understudied. To address this gap, this study aimed to investigate the angiogenic effects of selected commercially available food seasonings using the chorioallantoic membrane (CAM) assay in fertilized duck embryos. Methods: Twenty fertilized duck eggs were randomly assigned to four groups: control, Product X, Product Y, and Product Z. A 10% aqueous extract of each seasoning was applied to the CAM of eggs in the respective treatment groups. Blood vessel formation and embryo viability were assessed after incubation. Results: A significant reduction in blood vessel development was observed in all treatment groups compared to the control (p = 0.00184). Product Z exhibited the strongest antiangiogenic effect (96.15% inhibition), followed by Product Y (81.65%) and Product X (65.14%). Correspondingly, embryo mortality rates increased in treatment groups, correlating with the degree of angiogenesis inhibition. Conclusions: The findings suggested that certain food seasonings, particularly those containing monosodium glutamate (MSG) and other additives may impair embryonic vascular development and pose potential embryotoxic risks. The CAM assay proved to be a reliable and ethical in vivo model for evaluating the biological impact of dietary compounds. Further studies are recommended to explore dose-dependent responses and histomorphological changes associated with these seasonings.