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Analyzing the locomotory gaitprint of Caenorhabditis elegans on the basis of empirical mode decomposition

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  • Li-Chun Lin
  • Han-Sheng Chuang

Abstract

The locomotory gait analysis of the microswimmer, Caenorhabditis elegans, is a commonly adopted approach for strain recognition and examination of phenotypic defects. Gait is also a visible behavioral expression of worms under external stimuli. This study developed an adaptive data analysis method based on empirical mode decomposition (EMD) to reveal the biological cues behind intricate motion. The method was used to classify the strains of worms according to their gaitprints (i.e., phenotypic traits of locomotion). First, a norm of the locomotory pattern was created from the worm of interest. The body curvature of the worm was decomposed into four intrinsic mode functions (IMFs). A radar chart showing correlations between the predefined database and measured worm was then obtained by dividing each IMF into three parts, namely, head, mid-body, and tail. A comprehensive resemblance score was estimated after k-means clustering. Simulated data that use sinusoidal waves were generated to assess the feasibility of the algorithm. Results suggested that temporal frequency is the major factor in the process. In practice, five worm strains, including wild-type N2, TJ356 (zIs356), CL2070 (dvIs70), CB0061 (dpy-5), and CL2120 (dvIs14), were investigated. The overall classification accuracy of the gaitprint analyses of all the strains reached nearly 89%. The method can also be extended to classify some motor neuron-related locomotory defects of C. elegans in the same fashion.

Suggested Citation

  • Li-Chun Lin & Han-Sheng Chuang, 2017. "Analyzing the locomotory gaitprint of Caenorhabditis elegans on the basis of empirical mode decomposition," PLOS ONE, Public Library of Science, vol. 12(7), pages 1-14, July.
    • Handle: RePEc:plo:pone00:0181469
    DOI: 10.1371/journal.pone.0181469
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    References listed on IDEAS

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    1. Greg J Stephens & Bethany Johnson-Kerner & William Bialek & William S Ryu, 2008. "Dimensionality and Dynamics in the Behavior of C. elegans," PLOS Computational Biology, Public Library of Science, vol. 4(4), pages 1-10, April.
    2. Yelena Koren & Raphael Sznitman & Paulo E Arratia & Christopher Carls & Predrag Krajacic & André E X Brown & Josué Sznitman, 2015. "Model-Independent Phenotyping of C. elegans Locomotion Using Scale-Invariant Feature Transform," PLOS ONE, Public Library of Science, vol. 10(3), pages 1-16, March.
    3. Christophe Restif & Carolina Ibáñez-Ventoso & Mehul M Vora & Suzhen Guo & Dimitris Metaxas & Monica Driscoll, 2014. "CeleST: Computer Vision Software for Quantitative Analysis of C. elegans Swim Behavior Reveals Novel Features of Locomotion," PLOS Computational Biology, Public Library of Science, vol. 10(7), pages 1-12, July.
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