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Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product

Author

Listed:
  • P. Konstantin Richter

    (Leipzig University)

  • Paula Blázquez-Sánchez

    (Leipzig University)

  • Ziyue Zhao

    (Leipzig University)

  • Felipe Engelberger

    (Leipzig University Medical School)

  • Christian Wiebeler

    (Leipzig University
    Leipzig University)

  • Georg Künze

    (Leipzig University Medical School)

  • Ronny Frank

    (Leipzig University)

  • Dana Krinke

    (Leipzig University)

  • Emanuele Frezzotti

    (University of Parma)

  • Yuliia Lihanova

    (Leipzig University)

  • Patricia Falkenstein

    (Leipzig University)

  • Jörg Matysik

    (Leipzig University)

  • Wolfgang Zimmermann

    (Leipzig University)

  • Norbert Sträter

    (Leipzig University)

  • Christian Sonnendecker

    (Leipzig University)

Abstract

The recently discovered metagenomic-derived polyester hydrolase PHL7 is able to efficiently degrade amorphous polyethylene terephthalate (PET) in post-consumer plastic waste. We present the cocrystal structure of this hydrolase with its hydrolysis product terephthalic acid and elucidate the influence of 17 single mutations on the PET-hydrolytic activity and thermal stability of PHL7. The substrate-binding mode of terephthalic acid is similar to that of the thermophilic polyester hydrolase LCC and deviates from the mesophilic IsPETase. The subsite I modifications L93F and Q95Y, derived from LCC, increased the thermal stability, while exchange of H185S, derived from IsPETase, reduced the stability of PHL7. The subsite II residue H130 is suggested to represent an adaptation for high thermal stability, whereas L210 emerged as the main contributor to the observed high PET-hydrolytic activity. Variant L210T showed significantly higher activity, achieving a degradation rate of 20 µm h−1 with amorphous PET films.

Suggested Citation

  • P. Konstantin Richter & Paula Blázquez-Sánchez & Ziyue Zhao & Felipe Engelberger & Christian Wiebeler & Georg Künze & Ronny Frank & Dana Krinke & Emanuele Frezzotti & Yuliia Lihanova & Patricia Falken, 2023. "Structure and function of the metagenomic plastic-degrading polyester hydrolase PHL7 bound to its product," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37415-x
    DOI: 10.1038/s41467-023-37415-x
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    References listed on IDEAS

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