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Extensive co-binding and rapid redistribution of NANOG and GATA6 during emergence of divergent lineages

Author

Listed:
  • Joyce J. Thompson

    (National Institutes of Health)

  • Daniel J. Lee

    (National Institutes of Health)

  • Apratim Mitra

    (National Institutes of Health)

  • Sarah Frail

    (National Institutes of Health)

  • Ryan K. Dale

    (National Institutes of Health)

  • Pedro P. Rocha

    (National Institutes of Health
    NIH)

Abstract

Fate-determining transcription factors (TFs) can promote lineage-restricted transcriptional programs from common progenitor states. The inner cell mass (ICM) of mouse blastocysts co-expresses the TFs NANOG and GATA6, which drive the bifurcation of the ICM into either the epiblast (Epi) or the primitive endoderm (PrE), respectively. Here, we induce GATA6 in embryonic stem cells–that also express NANOG–to characterize how a state of co-expression of opposing TFs resolves into divergent lineages. Surprisingly, we find that GATA6 and NANOG co-bind at the vast majority of Epi and PrE enhancers, a phenomenon we also observe in blastocysts. The co-bound state is followed by eviction and repression of Epi TFs, and quick remodeling of chromatin and enhancer-promoter contacts thus establishing the PrE lineage while repressing the Epi fate. We propose that co-binding of GATA6 and NANOG at shared enhancers maintains ICM plasticity and promotes the rapid establishment of Epi- and PrE-specific transcriptional programs.

Suggested Citation

  • Joyce J. Thompson & Daniel J. Lee & Apratim Mitra & Sarah Frail & Ryan K. Dale & Pedro P. Rocha, 2022. "Extensive co-binding and rapid redistribution of NANOG and GATA6 during emergence of divergent lineages," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31938-5
    DOI: 10.1038/s41467-022-31938-5
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    2. Takeshi Katsuda & Jonathan H. Sussman & Kenji Ito & Andrew Katznelson & Salina Yuan & Naomi Takenaka & Jinyang Li & Allyson J. Merrell & Hector Cure & Qinglan Li & Reyaz Ur Rasool & Irfan A. Asangani , 2024. "Cellular reprogramming in vivo initiated by SOX4 pioneer factor activity," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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