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Inducible and reversible RNA N6-methyladenosine editing

Author

Listed:
  • Huaxia Shi

    (Case Western Reserve University)

  • Ying Xu

    (Case Western Reserve University)

  • Na Tian

    (Case Western Reserve University)

  • Ming Yang

    (Case Western Reserve University)

  • Fu-Sen Liang

    (Case Western Reserve University)

Abstract

RNA modifications, including N6-methyladenosine (m6A), have been reported to regulate fundamental RNA processes and properties, and directly linked to various human diseases. Methods enabling temporal and transcript/locus-specific editing of specific RNA modifications are essential, but still limited, to dissect the dynamic and context-dependent functions of these epigenetic modifications. Here, we develop a chemically inducible and reversible RNA m6A modification editing platform integrating chemically induced proximity (CIP) and CRISPR methods. We show that m6A editing can be temporally controlled at specific sites of individual RNA transcripts by the addition or removal of the CIP inducer, abscisic acid (ABA), in the system. By incorporating a photo-caged ABA, a light-controlled version of m6A editing platform can be developed. We expect that this platform and strategy can be generally applied to edit other RNA modifications in addition to m6A.

Suggested Citation

  • Huaxia Shi & Ying Xu & Na Tian & Ming Yang & Fu-Sen Liang, 2022. "Inducible and reversible RNA N6-methyladenosine editing," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29665-y
    DOI: 10.1038/s41467-022-29665-y
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    References listed on IDEAS

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