IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v5y2015i2p155-169d47375.html
   My bibliography  Save this article

Effect of Ozone Treatment on Inactivation of Escherichia coli and Listeria sp. on Spinach

Author

Listed:
  • Shreya Wani

    (School of Biology, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK)

  • Jagpreet K. Maker

    (School of Biology, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK)

  • Joseph R. Thompson

    (School of Biology, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK)

  • Jeremy Barnes

    (School of Biology, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK)

  • Ian Singleton

    (School of Biology, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK)

Abstract

The efficacy of “gaseous” ozone in reducing numbers and re-growth of food-borne pathogens, ( Escherichia coli and Listeria spp.), on leafy salads was investigated using spinach. A preliminary in vivo study showed 1-log reduction in six strains of E. coli and two species of Listeria spp. on spinach exposed to 1 ppm ozone for 10 min. A range of ozone treatments were explored to deliver optimal bacterial inactivation while maintaining the visual appearance (color) of produce. Exposure to a higher ozone concentration for a shorter duration (10 ppm for 2 min) significantly reduced E. coli and Listeria spp. viable counts by 1-log and the pathogens did not re-grow following treatment (over a nine-day storage period). Impacts of 1 and 10 ppm ozone treatments were not significantly different. Approximately 10% of the pathogen population was resistant to ozone treatment. We hypothesized that cell age may be one of several factors responsible for variation in ozone resistance. E. coli cells from older colonies demonstrated higher ozone resistance in subsequent experiments. Overall, we speculate that gaseous ozone treatment constitutes the basis for an alternative customer-friendly method to reduce food pathogen contamination of leafy produce and is worth exploring on a pilot-scale in an industrial setting.

Suggested Citation

  • Shreya Wani & Jagpreet K. Maker & Joseph R. Thompson & Jeremy Barnes & Ian Singleton, 2015. "Effect of Ozone Treatment on Inactivation of Escherichia coli and Listeria sp. on Spinach," Agriculture, MDPI, vol. 5(2), pages 1-15, March.
    • Handle: RePEc:gam:jagris:v:5:y:2015:i:2:p:155-169:d:47375
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/5/2/155/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/5/2/155/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. anonymous, 2000. "The brave new world of agriculture," EconSouth, Federal Reserve Bank of Atlanta, vol. 2(Q3), pages 8-13.
    2. Unknown, 2000. "Quality assurance in agricultural produce," ACIAR Proceedings Series 135369, Australian Centre for International Agricultural Research.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ameer Megahed & Brian Aldridge & James Lowe, 2018. "The microbial killing capacity of aqueous and gaseous ozone on different surfaces contaminated with dairy cattle manure," PLOS ONE, Public Library of Science, vol. 13(5), pages 1-22, May.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Kevin D. Welch & Daniel Cook & Benedict T. Green & Dale R. Gardner & James A. Pfister & Tara G. McDaneld & Kip E. Panter, 2015. "Adverse Effects of Larkspur ( Delphinium spp.) on Cattle," Agriculture, MDPI, vol. 5(3), pages 1-19, July.
    2. Chandran M. Ajila & Saurabh J. Sarma & Satinder K. Brar & Stephane Godbout & Michel Cote & Frederic Guay & Mausam Verma & Jose R. Valéro, 2015. "Fermented Apple Pomace as a Feed Additive to Enhance Growth Performance of Growing Pigs and Its Effects on Emissions," Agriculture, MDPI, vol. 5(2), pages 1-17, June.
    3. Masahiko Hirata, 2015. "Linking Management, Environment and Morphogenetic and Structural Components of a Sward for Simulating Tiller Density Dynamics in Bahiagrass ( Paspalum notatum )," Agriculture, MDPI, vol. 5(2), pages 1-14, June.
    4. Samphantharak, Krislert & Townsend, Robert M., 2012. "Measuring the return on household enterprise: What matters most for whom?," Journal of Development Economics, Elsevier, vol. 98(1), pages 58-70.
    5. Elke Bauriegel & Werner B. Herppich, 2014. "Hyperspectral and Chlorophyll Fluorescence Imaging for Early Detection of Plant Diseases, with Special Reference to Fusarium spec. Infections on Wheat," Agriculture, MDPI, vol. 4(1), pages 1-26, March.
    6. Zaumanis, Martins & Mallick, Rajib B. & Frank, Robert, 2014. "100% recycled hot mix asphalt: A review and analysis," Resources, Conservation & Recycling, Elsevier, vol. 92(C), pages 230-245.
    7. Hengyun Ma & Wei Liu & Les Oxley, 2012. "Productivity Growth And Policy Implications For China'S Dairy Farms," China Economic Policy Review (CEPR), World Scientific Publishing Co. Pte. Ltd., vol. 1(01), pages 1-20.
    8. Esther Mayer-Miebach & Marta Adamiuk & Diana Behsnilian, 2012. "Stability of Chokeberry Bioactive Polyphenols during Juice Processing and Stabilization of a Polyphenol-Rich Material from the By-Product," Agriculture, MDPI, vol. 2(3), pages 1-15, September.
    9. Haley, Usha C. V., 2003. "Assessing and controlling business risks in China," Journal of International Management, Elsevier, vol. 9(3), pages 237-252.
    10. Aoife L. McCarthy & Yvonne C. O'Callaghan & Nora M. O'Brien, 2013. "Protein Hydrolysates from Agricultural Crops—Bioactivity and Potential for Functional Food Development," Agriculture, MDPI, vol. 3(1), pages 1-19, February.
    11. Lange Bastian & Suwala Lech & Power Dominic, 2014. "Geographies of field-configuring events," ZFW – Advances in Economic Geography, De Gruyter, vol. 58(1), pages 187-201, October.
    12. Shih, Han M., 2014. "Migrating product structure bill of materials Excel files to STEP PDM implementation," International Journal of Information Management, Elsevier, vol. 34(4), pages 489-516.
    13. Kristin M. Trippe & Stephen M. Griffith & Gary M. Banowetz & Gerald W. Whitaker, 2015. "Biochars Derived from Gasified Feedstocks Increase the Growth and Improve Nutrient Acquisition of Triticum aestivum (L.) Grown in Agricultural Alfisols," Agriculture, MDPI, vol. 5(3), pages 1-14, August.
    14. Niklaus Lehmann & Robert Finger & Tommy Klein & Pierluigi Calanca, 2013. "Sample Size Requirements for Assessing Statistical Moments of Simulated Crop Yield Distributions," Agriculture, MDPI, vol. 3(2), pages 1-11, April.
    15. Nguyen, V.H. & Llewellyn, Rick S. & Miyan, M.S., 2007. "Explaining adoption of durum wheat in Western Australia," Australasian Agribusiness Review, University of Melbourne, Department of Agriculture and Food Systems, vol. 15.
    16. Satwinder Singh & Chima Mordi & Chinonye Okafor & Ruth Simpson, 2010. "Challenges In Female Entrepreneurial Development — A Case Analysis Of Nigerian Entrepreneurs," Journal of Enterprising Culture (JEC), World Scientific Publishing Co. Pte. Ltd., vol. 18(04), pages 435-460.
    17. Joel P. Hague & Stephen L. Dellaporta & Maria A. Moreno & Chip Longo & Kimberly Nelson & Albert P. Kausch, 2012. "Pollen Sterility—A Promising Approach to Gene Confinement and Breeding for Genetically Modified Bioenergy Crops," Agriculture, MDPI, vol. 2(4), pages 1-21, October.
    18. Burkard Kautz & Mauricio Hunsche & Georg Noga, 2014. "Salinity-Induced Changes of Multiparametric Fluorescence Indices of Tomato Leaves," Agriculture, MDPI, vol. 4(2), pages 1-15, April.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:5:y:2015:i:2:p:155-169:d:47375. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.