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Development of KASP and SSR Markers for PmQ , a Recessive Gene Conferring Powdery Mildew Resistance in Wheat Landrace Qingxinmai

Author

Listed:
  • Habteab Goitom Gebremedhin

    (The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    These authors contributed equally to this work.)

  • Yahui Li

    (College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China
    These authors contributed equally to this work.)

  • Jinghuang Hu

    (The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Dan Qiu

    (The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China)

  • Qiuhong Wu

    (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China)

  • Hongjun Zhang

    (The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Li Yang

    (The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Yang Zhou

    (The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

  • Yijun Zhou

    (College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China)

  • Zhiyong Liu

    (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China)

  • Peng Zhang

    (School of Life and Environmental Sciences, Plant Breeding Institute, University of Sydney, Cobbitty, NSW 2570, Australia)

  • Hongjie Li

    (The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Abstract

A recessive gene PmQ conferring powdery mildew resistance was previously localized on the long arm of chromosome 2B in winter wheat landrace Qingxinmai. Breeder-friendly molecular markers are necessary for introgressing this gene into adapted wheat backgrounds for developing disease-resistant wheat cultivars. Three Kompetitive allele-specific PCR (KASP) markers were developed and validated based on SNP variants detected by Bulked segregant analysis-RNA-Seq (BSR-Seq) analysis using a recombinant inbred population derived from cross Qingxinmai × 041133. Two polymorphic SSR markers were also developed from the motifs in the Chinese Spring reference genome sequences of the target genomic region. Those markers were incorporated into a more saturated genetic linkage map for PmQ . The two flanking markers, Xicsq405 and Xicsk18 , are 1.1 and 0.9 cM from PmQ , respectively. The KASP marker Xicsk19 produced unique amplification pattern in 158 out of 160 wheat cultivars or breeding lines. This marker with the gene-linked SSR markers Xicsqc , Xicsqd and Xicsq405 provides an efficient means in molecular marker-assisted selection for PmQ in wheat breeding. The corresponding genomic region of PmQ in the Chinese Spring reference genome has a conserved synteny with the genomes of sequenced wheat cultivars and Triticum durum , T. diccocoides , T. uratu , and barley. The annotation of the two genes, TraesCS2B01G517400.1 and TraesCS2B01G517700.1 , associated with plant defense against pathogens placed a start for cloning PmQ .

Suggested Citation

  • Habteab Goitom Gebremedhin & Yahui Li & Jinghuang Hu & Dan Qiu & Qiuhong Wu & Hongjun Zhang & Li Yang & Yang Zhou & Yijun Zhou & Zhiyong Liu & Peng Zhang & Hongjie Li, 2022. "Development of KASP and SSR Markers for PmQ , a Recessive Gene Conferring Powdery Mildew Resistance in Wheat Landrace Qingxinmai," Agriculture, MDPI, vol. 12(9), pages 1-10, August.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:9:p:1344-:d:902517
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    References listed on IDEAS

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    1. Martin Mascher & Heidrun Gundlach & Axel Himmelbach & Sebastian Beier & Sven O. Twardziok & Thomas Wicker & Volodymyr Radchuk & Christoph Dockter & Pete E. Hedley & Joanne Russell & Micha Bayer & Luke, 2017. "A chromosome conformation capture ordered sequence of the barley genome," Nature, Nature, vol. 544(7651), pages 427-433, April.
    2. Sean Walkowiak & Liangliang Gao & Cecile Monat & Georg Haberer & Mulualem T. Kassa & Jemima Brinton & Ricardo H. Ramirez-Gonzalez & Markus C. Kolodziej & Emily Delorean & Dinushika Thambugala & Valent, 2020. "Multiple wheat genomes reveal global variation in modern breeding," Nature, Nature, vol. 588(7837), pages 277-283, December.
    3. Hong-Qing Ling & Bin Ma & Xiaoli Shi & Hui Liu & Lingli Dong & Hua Sun & Yinghao Cao & Qiang Gao & Shusong Zheng & Ye Li & Ying Yu & Huilong Du & Ming Qi & Yan Li & Hongwei Lu & Hua Yu & Yan Cui & Nin, 2018. "Genome sequence of the progenitor of wheat A subgenome Triticum urartu," Nature, Nature, vol. 557(7705), pages 424-428, May.
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