IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v55y2010i8id89-2009-cjas.html
   My bibliography  Save this article

TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene

Author

Listed:
  • I. Manga

    (Agriresearch Rapotín Ltd., Rapotín, Czech Republic)

  • J. Dvořák

    (Department of Animal Morphology, Physiology and Genetics, Mendel University in Brno, Brno, Czech Republic)

Abstract

Alleles A1 and A2 of the Bos taurus CSN2 gene are the most frequent in a number of dairy cattle breeds. In this study, a new allelic discrimination assay using TaqMan fluorogenic probes was developed to detect single nucleotide substitution characterizing the A1/A2 alleles of the CSN2 gene. The method was validated using DNA samples of known genotypes with different concentrations and the results were compared with those for the commonly used problematic ACRS-PCR. We found the TaqMan method to be more effective, 100% reliable and hundred times more sensitive for testing the CSN2 genetic marker in cattle than the ACRS-PCR. As it enabled a rapid analysis of a large number of DNA samples in uniform format without previous DNA quality assessment and without the requirement for post-amplification manipulations, it presents an effective tool for the analysis of large-scale sample sets. The method was applied for testing on a sample of 120 Czech Holstein dairy cows. The observed relative genotype and allele frequencies were as follows: A1A1-0.20, A1A2-0.51, A2A2-0.29; A1-0.45, A2-0.55.

Suggested Citation

  • I. Manga & J. Dvořák, 2010. "TaqMan allelic discrimination assay for A1 and A2 alleles of the bovine CSN2 gene," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(8), pages 307-312.
    • Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:8:id:89-2009-cjas
    DOI: 10.17221/89/2009-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/89/2009-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/89/2009-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/89/2009-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. J. Kučerová & A. Matějíček & O.M. Jandurová & P. Sørensen & E. Němcová & M. Štípková & T. Kott & J. Bouška & J. Frelich, 2006. "Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(6), pages 241-247.
    2. E. Hradecká & J. Čítek & L. Panicke & V. Řehout & L. Hanusová, 2008. "The relation of GH1, GHR and DGAT1 polymorphisms with estimated breeding values for milk production traits of German Holstein sires," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 53(6), pages 238-246.
    3. J. Čítek & L. Panicke & V. Řehout & H. Procházková, 2006. "Study of genetic distances between cattle breeds of Central Europe," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(10), pages 429-436.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. J. Čítek & V. Řehout & L. Hanusová & A. Míková & I. Jašková, 2011. "Polymorphisms in CGIL4, breeding value for somatic cell count and resistance to mastitis," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(7), pages 301-304.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. J. Stevanovic & Z. Stanimirovic & V. Dimitrijevic & M. Maletic, 2010. "Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(6), pages 221-226.
    2. J. Čítek & L. Panicke & V. Řehout & H. Procházková, 2006. "Study of genetic distances between cattle breeds of Central Europe," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 51(10), pages 429-436.
    3. T. Szulc & M. Pawelska-Góral & K. Hajduk, 2009. "Effect of wheat gluten and extracted, protected soybean meal addition to the diet of cows with different beta-lactoglobulin genotypes on the composition and physical properties of milk," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 54(8), pages 341-348.
    4. L. Hanusová & A. Míková & L. Večerek & D. Schroeffelová & V. Řehout & L. Tothová & K. Vernerová & B. Hosnedlová & J. Čítek, 2014. "Effect of DGAT1 polymorphisms on the estimated breeding values of Czech Simmental sires," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 59(8), pages 365-373.
    5. A. Matějíček & J. Matějíčková & E. Němcová & O.M. Jandurová & M. Štípková & J. Bouška & J. Frelich, 2007. "Joint effects of CSN3 and LGB genotypes and their relation to breeding values of milk production parameters in Czech Fleckvieh," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(4), pages 83-87.
    6. M. Szewczuk & M. Bajurna & S. Zych & W. Kruszyński, 2013. "Association of insulin-like growth factor I gene polymorphisms (IGF1/TasI and IGF1/SnaBI) with the growth and subsequent milk yield of Polish Holstein-Friesian heifers," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(9), pages 404-411.
    7. H. Kulig & I. Kowalewska-Łuczak & M. Kmieć & K. Wojdak-Maksymiec, 2010. "ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traitsin Jersey cows," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(11), pages 463-467.
    8. A. Stachurska & A. Nogaj & A. Brodacki & J. Nogaj & J. Batkowska, 2014. "Genetic distances between horse breeds in Poland estimated according to blood protein polymorphism," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 59(6), pages 257-267.
    9. A. Abdolmohammadi & H. Atashi & P. Zamani & C. Bottema, 2011. "High resolution melting as an alternative method to genotype diacylglycerol O-acyltransferase 1 (DGAT1) K232A polymorphism in cattle," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 56(8), pages 370-376.
    10. I. Kowalewska-Łuczak & H. Kulig & M. Kmieć, 2010. "Associations between the bovine PPARGC1A gene and milk production traits," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(5), pages 195-199.

    More about this item

    Keywords

    allelic discrimination; CSN2; alleles A1 and A2; cattle;
    All these keywords.

    JEL classification:

    • A1 - General Economics and Teaching - - General Economics
    • A2 - General Economics and Teaching - - Economic Education and Teaching of Economics

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:55:y:2010:i:8:id:89-2009-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.