IDEAS home Printed from https://ideas.repec.org/a/wly/greenh/v8y2018i2p279-291.html
   My bibliography  Save this article

Characterization of technical grade carbonic anhydrase as biocatalyst for CO2 capture in potassium carbonate solutions

Author

Listed:
  • Sara Peirce
  • Rosa Perfetto
  • Maria Elena Russo
  • Clemente Capasso
  • Mosè Rossi
  • Piero Salatino
  • Antonio Marzocchella

Abstract

Reactive absorption promoted by carbonic anhydrase (CA, E.C. 4.2.1.1.) catalysis has been proposed for CO2 capture from exhaust gas as an alternative to the reactive absorption in amine solutions. Potassium carbonate solutions allow feasible CO2 capture assisted by CA. This paper reports on the characterization of a CA form supplied by Novozymes as a catalyst for CO2 capture in K2CO3 solutions at operating conditions relevant for industrial processes (2–3 M K2CO3, 298–313 K, 0–40% carbonate to bicarbonate conversion). CO2 absorption tests were carried out in a batch†stirred cell apparatus at constant gas volume and temperature by measuring gas pressure decay. The enhancement ratio between CO2 absorption rate with and without the enzyme at biocatalyst concentration above 0.3 kg m−3 ranged between 5 and 8 depending on the solvent composition and temperature. Active enzyme aggregates formed at protein concentration larger than 0.3 kg/m3 and their contribution to the absorption rate enhancement was remarkable. Assessment of enzyme kinetics in homogeneous solutions at enzyme concentration lower than 0.018 kg·m−3 showed that kcat/KM increased with both temperature and salt concentration. kcat/KM ranged between 9.0·103 and 7.0·104 m3·kg−1·s−1 in agreement with literature data on carbonic anhydrase kinetics. Reliability of the adopted method for kinetic characterization was confirmed. The activity of enzyme aggregates formed in the carbonate solvent was verified and CA aggregates can be used to select the proper CA based biocatalyst for CO2 capture application as an alternative to free and immobilized enzyme. © 2017 Society of Chemical Industry and John Wiley & Sons, Ltd.

Suggested Citation

  • Sara Peirce & Rosa Perfetto & Maria Elena Russo & Clemente Capasso & Mosè Rossi & Piero Salatino & Antonio Marzocchella, 2018. "Characterization of technical grade carbonic anhydrase as biocatalyst for CO2 capture in potassium carbonate solutions," Greenhouse Gases: Science and Technology, Blackwell Publishing, vol. 8(2), pages 279-291, April.
    • Handle: RePEc:wly:greenh:v:8:y:2018:i:2:p:279-291
    DOI: 10.1002/ghg.1738
    as

    Download full text from publisher

    File URL: https://doi.org/10.1002/ghg.1738
    Download Restriction: no
    File URL: https://libkey.io/10.1002/ghg.1738?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Kunze, Anna-Katharina & Dojchinov, Greg & Haritos, Victoria S. & Lutze, Philip, 2015. "Reactive absorption of CO2 into enzyme accelerated solvents: From laboratory to pilot scale," Applied Energy, Elsevier, vol. 156(C), pages 676-685.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Wang, Fu & Zhao, Jun & Li, Hailong & Deng, Shuai & Yan, Jinyue, 2017. "Preliminary experimental study of post-combustion carbon capture integrated with solar thermal collectors," Applied Energy, Elsevier, vol. 185(P2), pages 1471-1480.
    2. Rongrong Zhai & Hongtao Liu & Hao Wu & Hai Yu & Yongping Yang, 2018. "Analysis of Integration of MEA-Based CO 2 Capture and Solar Energy System for Coal-Based Power Plants Based on Thermo-Economic Structural Theory," Energies, MDPI, vol. 11(5), pages 1-30, May.
    3. Qi, Guojie & Liu, Kun & House, Alan & Salmon, Sonja & Ambedkar, Balraj & Frimpong, Reynolds A. & Remias, Joseph E. & Liu, Kunlei, 2018. "Laboratory to bench-scale evaluation of an integrated CO2 capture system using a thermostable carbonic anhydrase promoted K2CO3 solvent with low temperature vacuum stripping," Applied Energy, Elsevier, vol. 209(C), pages 180-189.
    4. Putta, Koteswara Rao & Tobiesen, Finn Andrew & Svendsen, Hallvard F. & Knuutila, Hanna K., 2017. "Applicability of enhancement factor models for CO2 absorption into aqueous MEA solutions," Applied Energy, Elsevier, vol. 206(C), pages 765-783.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:wly:greenh:v:8:y:2018:i:2:p:279-291. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Wiley Content Delivery (email available below). General contact details of provider: https://doi.org/10.1002/(ISSN)2152-3878 .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.