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Targeted nanodiamonds for identification of subcellular protein assemblies in mammalian cells

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  • Michael P Lake
  • Louis-S Bouchard

Abstract

Transmission electron microscopy (TEM) can be used to successfully determine the structures of proteins. However, such studies are typically done ex situ after extraction of the protein from the cellular environment. Here we describe an application for nanodiamonds as targeted intensity contrast labels in biological TEM, using the nuclear pore complex (NPC) as a model macroassembly. We demonstrate that delivery of antibody-conjugated nanodiamonds to live mammalian cells using maltotriose-conjugated polypropylenimine dendrimers results in efficient localization of nanodiamonds to the intended cellular target. We further identify signatures of nanodiamonds under TEM that allow for unambiguous identification of individual nanodiamonds from a resin-embedded, OsO4-stained environment. This is the first demonstration of nanodiamonds as labels for nanoscale TEM-based identification of subcellular protein assemblies. These results, combined with the unique fluorescence properties and biocompatibility of nanodiamonds, represent an important step toward the use of nanodiamonds as markers for correlated optical/electron bioimaging.

Suggested Citation

  • Michael P Lake & Louis-S Bouchard, 2017. "Targeted nanodiamonds for identification of subcellular protein assemblies in mammalian cells," PLOS ONE, Public Library of Science, vol. 12(6), pages 1-18, June.
    • Handle: RePEc:plo:pone00:0179295
    DOI: 10.1371/journal.pone.0179295
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    1. Wei‐Kei Shiue & Lee J. Bain, 1982. "Experiment Size and Power Comparisons for Two‐Sample Poisson Tests," Journal of the Royal Statistical Society Series C, Royal Statistical Society, vol. 31(2), pages 130-134, June.
    2. D. Le Sage & K. Arai & D. R. Glenn & S. J. DeVience & L. M. Pham & L. Rahn-Lee & M. D. Lukin & A. Yacoby & A. Komeili & R. L. Walsworth, 2013. "Optical magnetic imaging of living cells," Nature, Nature, vol. 496(7446), pages 486-489, April.
    3. Alexander von Appen & Jan Kosinski & Lenore Sparks & Alessandro Ori & Amanda L. DiGuilio & Benjamin Vollmer & Marie-Therese Mackmull & Niccolo Banterle & Luca Parca & Panagiotis Kastritis & Katarzyna , 2015. "In situ structural analysis of the human nuclear pore complex," Nature, Nature, vol. 526(7571), pages 140-143, October.
    4. G. Kucsko & P. C. Maurer & N. Y. Yao & M. Kubo & H. J. Noh & P. K. Lo & H. Park & M. D. Lukin, 2013. "Nanometre-scale thermometry in a living cell," Nature, Nature, vol. 500(7460), pages 54-58, August.
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