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Temporospatial hierarchy and allele-specific expression of zygotic genome activation revealed by distant interspecific urochordate hybrids

Author

Listed:
  • Jiankai Wei

    (Ocean University of China
    Qingdao Marine Science and Technology Center
    Ocean University of China)

  • Wei Zhang

    (Ocean University of China)

  • An Jiang

    (Ocean University of China)

  • Hongzhe Peng

    (Ocean University of China)

  • Quanyong Zhang

    (Kunming University of Science and Technology)

  • Yuting Li

    (Ocean University of China)

  • Jianqing Bi

    (Ocean University of China)

  • Linting Wang

    (Chinese Academy of Sciences)

  • Penghui Liu

    (Ocean University of China)

  • Jing Wang

    (Ocean University of China)

  • Yonghang Ge

    (Ocean University of China)

  • Liya Zhang

    (Kunming University of Science and Technology)

  • Haiyan Yu

    (Ocean University of China)

  • Lei Li

    (Chinese Academy of Sciences)

  • Shi Wang

    (Ocean University of China
    Qingdao Marine Science and Technology Center)

  • Liang Leng

    (Chengdu University of Traditional Chinese Medicine)

  • Kai Chen

    (Kunming University of Science and Technology
    Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou))

  • Bo Dong

    (Ocean University of China
    Qingdao Marine Science and Technology Center
    Ocean University of China)

Abstract

Zygotic genome activation (ZGA) is a universal process in early embryogenesis of metazoan, when the quiescent zygotic nucleus initiates global transcription. However, the mechanisms related to massive genome activation and allele-specific expression (ASE) remain not well understood. Here, we develop hybrids from two deeply diverged (120 Mya) ascidian species to symmetrically document the dynamics of ZGA. We identify two coordinated ZGA waves represent early developmental and housekeeping gene reactivation, respectively. Single-cell RNA sequencing reveals that the major expression wave exhibits spatial heterogeneity and significantly correlates with cell fate. Moreover, allele-specific expression occurs in a species- rather than parent-related manner, demonstrating the divergence of cis-regulatory elements between the two species. These findings provide insights into ZGA in chordates.

Suggested Citation

  • Jiankai Wei & Wei Zhang & An Jiang & Hongzhe Peng & Quanyong Zhang & Yuting Li & Jianqing Bi & Linting Wang & Penghui Liu & Jing Wang & Yonghang Ge & Liya Zhang & Haiyan Yu & Lei Li & Shi Wang & Liang, 2024. "Temporospatial hierarchy and allele-specific expression of zygotic genome activation revealed by distant interspecific urochordate hybrids," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46780-0
    DOI: 10.1038/s41467-024-46780-0
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    References listed on IDEAS

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