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Proximity extracellular protein-protein interaction analysis of EGFR using AirID-conjugated fragment of antigen binding

Author

Listed:
  • Kohdai Yamada

    (Ehime University)

  • Ryouhei Shioya

    (Ehime University)

  • Kohei Nishino

    (Tokushima University)

  • Hirotake Furihata

    (Ehime University)

  • Atsushi Hijikata

    (Tokyo University of Pharmacy and Life Sciences)

  • Mika K. Kaneko

    (Tohoku University Graduate School of Medicine
    Tohoku University Graduate School of Medicine)

  • Yukinari Kato

    (Tohoku University Graduate School of Medicine
    Tohoku University Graduate School of Medicine)

  • Tsuyoshi Shirai

    (Nagahama Institute of BioScience and Technology)

  • Hidetaka Kosako

    (Tokushima University)

  • Tatsuya Sawasaki

    (Ehime University)

Abstract

Receptor proteins, such as epidermal growth factor receptor (EGFR), interact with other proteins in the extracellular region of the cell membrane to drive intracellular signalling. Therefore, analysis of extracellular protein-protein interactions (exPPIs) is important for understanding the biological function of receptor proteins. Here, we present an approach using a proximity biotinylation enzyme (AirID) fusion fragment of antigen binding (FabID) to analyse the proximity exPPIs of EGFR. AirID was C-terminally fused to the Fab fragment against EGFR (EGFR-FabID), which could then biotinylate the extracellular region of EGFR in several cell lines. Liquid Chromatography-Mass Spectrometry (LC-MS/MS) analysis indicated that many known EGFR interactors were identified as proximity exPPIs, along with many unknown candidate interactors, using EGFR-FabID. Interestingly, these proximity exPPIs were influenced by treatment with EGF ligand and its specific kinase inhibitor, gefitinib. These results indicate that FabID provides accurate proximity exPPI analysis of target receptor proteins on cell membranes with ligand and drug responses.

Suggested Citation

  • Kohdai Yamada & Ryouhei Shioya & Kohei Nishino & Hirotake Furihata & Atsushi Hijikata & Mika K. Kaneko & Yukinari Kato & Tsuyoshi Shirai & Hidetaka Kosako & Tatsuya Sawasaki, 2023. "Proximity extracellular protein-protein interaction analysis of EGFR using AirID-conjugated fragment of antigen binding," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43931-7
    DOI: 10.1038/s41467-023-43931-7
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    References listed on IDEAS

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