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Klebsiella pneumoniae clinical isolates with features of both multidrug-resistance and hypervirulence have unexpectedly low virulence

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  • Travis J. Kochan

    (Laboratory of Respiratory and Special Pathogens, Division of Bacterial, Parasitic, and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration
    Northwestern University)

  • Sophia H. Nozick

    (Northwestern University)

  • Aliki Valdes

    (Northwestern University)

  • Sumitra D. Mitra

    (Northwestern University)

  • Bettina H. Cheung

    (Northwestern University)

  • Marine Lebrun-Corbin

    (Northwestern University)

  • Rachel L. Medernach

    (Northwestern University
    Northwestern University)

  • Madeleine B. Vessely

    (Northwestern University)

  • Jori O. Mills

    (Northwestern University)

  • Christopher M. R. Axline

    (Northwestern University)

  • Julia A. Nelson

    (Northwestern University)

  • Ethan M. VanGosen

    (Northwestern University)

  • Timothy J. Ward

    (Northwestern University)

  • Egon A. Ozer

    (Northwestern University)

  • David Duin

    (University of North Carolina)

  • Liang Chen

    (Center for Discovery and Innovation, Hackensack Meridian Health)

  • Barry N. Kreiswirth

    (Center for Discovery and Innovation, Hackensack Meridian Health)

  • S. Wesley Long

    (Houston Methodist Hospital Research Institute)

  • James M. Musser

    (Houston Methodist Hospital Research Institute)

  • Zackery P. Bulman

    (University of Illinois at Chicago)

  • Richard G. Wunderink

    (Northwestern University
    Northwestern University)

  • Alan R. Hauser

    (Northwestern University
    Northwestern University)

Abstract

Klebsiella pneumoniae has been classified into two types, classical K. pneumoniae (cKP) and hypervirulent K. pneumoniae (hvKP). cKP isolates are highly diverse and important causes of nosocomial infections; they include globally disseminated antibiotic-resistant clones. hvKP isolates are sensitive to most antibiotics but are highly virulent, causing community-acquired infections in healthy individuals. The virulence phenotype of hvKP is associated with pathogenicity loci responsible for siderophore and hypermucoid capsule production. Recently, convergent strains of K. pneumoniae, which possess features of both cKP and hvKP, have emerged and are cause of much concern. Here, we screen the genomes of 2,608 multidrug-resistant K. pneumoniae isolates from the United States and identify 47 convergent isolates. We perform phenotypic and genomic characterization of 12 representative isolates. These 12 convergent isolates contain a variety of antimicrobial resistance plasmids and virulence plasmids. Most convergent isolates contain aerobactin biosynthesis genes and produce more siderophores than cKP isolates but not more capsule. Unexpectedly, only 1 of the 12 tested convergent isolates has a level of virulence consistent with hvKP isolates in a murine pneumonia model. These findings suggest that additional studies should be performed to clarify whether convergent strains are indeed more virulent than cKP in mouse and human infections.

Suggested Citation

  • Travis J. Kochan & Sophia H. Nozick & Aliki Valdes & Sumitra D. Mitra & Bettina H. Cheung & Marine Lebrun-Corbin & Rachel L. Medernach & Madeleine B. Vessely & Jori O. Mills & Christopher M. R. Axline, 2023. "Klebsiella pneumoniae clinical isolates with features of both multidrug-resistance and hypervirulence have unexpectedly low virulence," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43802-1
    DOI: 10.1038/s41467-023-43802-1
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    References listed on IDEAS

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    1. Christian Ritz & Florent Baty & Jens C Streibig & Daniel Gerhard, 2015. "Dose-Response Analysis Using R," PLOS ONE, Public Library of Science, vol. 10(12), pages 1-13, December.
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