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Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit

Author

Listed:
  • Haidai Hu

    (University of Copenhagen)

  • Philipp F. Popp

    (Humboldt-Universität zu Berlin)

  • Mònica Santiveri

    (University of Copenhagen)

  • Aritz Roa-Eguiara

    (University of Copenhagen)

  • Yumeng Yan

    (University of Copenhagen)

  • Freddie J. O. Martin

    (University of Copenhagen)

  • Zheyi Liu

    (Zhejiang University
    International Campus of Zhejiang University)

  • Navish Wadhwa

    (Arizona State University
    Arizona State University)

  • Yong Wang

    (Zhejiang University
    International Campus of Zhejiang University)

  • Marc Erhardt

    (Humboldt-Universität zu Berlin
    Max Planck Unit for the Science of Pathogens)

  • Nicholas M. I. Taylor

    (University of Copenhagen)

Abstract

Bacteria swim using a flagellar motor that is powered by stator units. Vibrio spp. are highly motile bacteria responsible for various human diseases, the polar flagella of which are exclusively driven by sodium-dependent stator units (PomAB). However, how ion selectivity is attained, how ion transport triggers the directional rotation of the stator unit, and how the stator unit is incorporated into the flagellar rotor remained largely unclear. Here, we have determined by cryo-electron microscopy the structure of Vibrio PomAB. The electrostatic potential map uncovers sodium binding sites, which together with functional experiments and molecular dynamics simulations, reveal a mechanism for ion translocation and selectivity. Bulky hydrophobic residues from PomA prime PomA for clockwise rotation. We propose that a dynamic helical motif in PomA regulates the distance between PomA subunit cytoplasmic domains, stator unit activation, and torque transmission. Together, our study provides mechanistic insights for understanding ion selectivity and rotor incorporation of the stator unit of the bacterial flagellum.

Suggested Citation

  • Haidai Hu & Philipp F. Popp & Mònica Santiveri & Aritz Roa-Eguiara & Yumeng Yan & Freddie J. O. Martin & Zheyi Liu & Navish Wadhwa & Yong Wang & Marc Erhardt & Nicholas M. I. Taylor, 2023. "Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39899-z
    DOI: 10.1038/s41467-023-39899-z
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