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Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction

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  • Miaowei Mao

    (East China University of Science and Technology
    East China University of Science and Technology
    University of Chinese Academy of Sciences, Chinese Academy of Sciences)

  • Yajie Qian

    (East China University of Science and Technology
    East China University of Science and Technology)

  • Wenyao Zhang

    (East China University of Science and Technology)

  • Siyu Zhou

    (East China University of Science and Technology
    East China University of Science and Technology)

  • Zefeng Wang

    (University of Chinese Academy of Sciences, Chinese Academy of Sciences)

  • Xianjun Chen

    (East China University of Science and Technology
    East China University of Science and Technology)

  • Yi Yang

    (East China University of Science and Technology
    East China University of Science and Technology)

Abstract

Optogenetics tools for precise temporal and spatial control of protein abundance are valuable in studying diverse complex biological processes. In the present study, we engineer a monomeric tag of stabilization upon light induction (SULI) for yeast and zebrafish based on a single light-oxygen-voltage domain from Neurospora crassa. Proteins of interest fused with SULI are stable upon light illumination but are readily degraded after transfer to dark conditions. SULI shows a high dynamic range and a high tolerance to fusion at different positions of the target protein. Further studies reveal that SULI-mediated degradation occurs through a lysine ubiquitination-independent proteasome pathway. We demonstrate the usefulness of SULI in controlling the cell cycle in yeast and regulating protein stability in zebrafish, respectively. Overall, our data indicate that SULI is a simple and robust tool to quantitatively and spatiotemporally modulate protein levels for biotechnological or biomedical applications.

Suggested Citation

  • Miaowei Mao & Yajie Qian & Wenyao Zhang & Siyu Zhou & Zefeng Wang & Xianjun Chen & Yi Yang, 2023. "Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37830-0
    DOI: 10.1038/s41467-023-37830-0
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    1. Andrew Fire & SiQun Xu & Mary K. Montgomery & Steven A. Kostas & Samuel E. Driver & Craig C. Mello, 1998. "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans," Nature, Nature, vol. 391(6669), pages 806-811, February.
    2. Fuun Kawano & Hideyuki Suzuki & Akihiro Furuya & Moritoshi Sato, 2015. "Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins," Nature Communications, Nature, vol. 6(1), pages 1-8, May.
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