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Structures of the CcmABCD heme release complex at multiple states

Author

Listed:
  • Jiao Li

    (Nanjing University of Chinese Medicine
    Yale University)

  • Wan Zheng

    (Nanjing University of Chinese Medicine)

  • Ming Gu

    (Nanjing University of Chinese Medicine)

  • Long Han

    (Yale University)

  • Yanmei Luo

    (Nanjing University of Chinese Medicine)

  • Koukou Yu

    (Nanjing University of Chinese Medicine)

  • Mengxin Sun

    (Nanjing University of Chinese Medicine)

  • Yuliang Zong

    (Nanjing University of Chinese Medicine)

  • Xiuxiu Ma

    (Nanjing University of Chinese Medicine)

  • Bing Liu

    (Nanjing University of Chinese Medicine)

  • Ethan P. Lowder

    (Washington University in St. Louis)

  • Deanna L. Mendez

    (Washington University in St. Louis)

  • Robert G. Kranz

    (Washington University in St. Louis)

  • Kai Zhang

    (Yale University)

  • Jiapeng Zhu

    (Nanjing University of Chinese Medicine)

Abstract

Cytochromes c use heme as a cofactor to carry electrons in respiration and photosynthesis. The cytochrome c maturation system I, consisting of eight membrane proteins (CcmABCDEFGH), results in the attachment of heme to cysteine residues of cytochrome c proteins. Since all c-type cytochromes are periplasmic, heme is first transported to a periplasmic heme chaperone, CcmE. A large membrane complex, CcmABCD has been proposed to carry out this transport and linkage to CcmE, yet the structural basis and mechanisms underlying the process are unknown. We describe high resolution cryo-EM structures of CcmABCD in an unbound form, in complex with inhibitor AMP-PNP, and in complex with ATP and heme. We locate the ATP-binding site in CcmA and the heme-binding site in CcmC. Based on our structures combined with functional studies, we propose a hypothetic model of heme trafficking, heme transfer to CcmE, and ATP-dependent release of holoCcmE from CcmABCD. CcmABCD represents an ABC transporter complex using the energy of ATP hydrolysis for the transfer of heme from one binding partner (CcmC) to another (CcmE).

Suggested Citation

  • Jiao Li & Wan Zheng & Ming Gu & Long Han & Yanmei Luo & Koukou Yu & Mengxin Sun & Yuliang Zong & Xiuxiu Ma & Bing Liu & Ethan P. Lowder & Deanna L. Mendez & Robert G. Kranz & Kai Zhang & Jiapeng Zhu, 2022. "Structures of the CcmABCD heme release complex at multiple states," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34136-5
    DOI: 10.1038/s41467-022-34136-5
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    1. Lorena Ilcu & Lukas Denkhaus & Anton Brausemann & Lin Zhang & Oliver Einsle, 2023. "Architecture of the Heme-translocating CcmABCD/E complex required for Cytochrome c maturation," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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