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Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P

Author

Listed:
  • Jiaqiang Zhu

    (University of Florida)

  • Wei Huang

    (Case Western Reserve University School of Medicine)

  • Jing Zhao

    (University of Florida)

  • Loc Huynh

    (University of Florida)

  • Derek J. Taylor

    (Case Western Reserve University School of Medicine
    Case Western Reserve University School of Medicine)

  • Michael E. Harris

    (University of Florida)

Abstract

Binding of precursor tRNAs (ptRNAs) by bacterial ribonuclease P (RNase P) involves an encounter complex (ES) that isomerizes to a catalytic conformation (ES*). However, the structures of intermediates and the conformational changes that occur during binding are poorly understood. Here, we show that pairing between the 5′ leader and 3′RCCA extending the acceptor stem of ptRNA inhibits ES* formation. Cryo-electron microscopy single particle analysis reveals a dynamic enzyme that becomes ordered upon formation of ES* in which extended acceptor stem pairing is unwound. Comparisons of structures with alternative ptRNAs reveals that once unwinding is completed RNase P primarily uses stacking interactions and shape complementarity to accommodate alternative sequences at its cleavage site. Our study reveals active site interactions and conformational changes that drive molecular recognition by RNase P and lays the foundation for understanding how binding interactions are linked to helix unwinding and catalysis.

Suggested Citation

  • Jiaqiang Zhu & Wei Huang & Jing Zhao & Loc Huynh & Derek J. Taylor & Michael E. Harris, 2022. "Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32843-7
    DOI: 10.1038/s41467-022-32843-7
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