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Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton

Author

Listed:
  • Yanchun You

    (Xiamen University)

  • Xueqiong Sun

    (Xiamen University)

  • Minglei Ma

    (Xiamen University)

  • Jiamin He

    (Xiamen University)

  • Ling Li

    (Xiamen University)

  • Felipe Wendt Porto

    (University of Connecticut)

  • Senjie Lin

    (Xiamen University
    University of Connecticut)

Abstract

Trypsin is best known as a digestive enzyme in animals, but remains unexplored in phytoplankton, the major primary producers in the ocean. Here we report the prevalence of trypsin genes in global ocean phytoplankton and significant influences of environmental nitrogen (N) and phosphorus (P) on their expression. Using CRISPR/Cas9 mediated-knockout and overexpression analyses, we further reveal that a trypsin in Phaeodactylum tricornutum (PtTryp2) functions to repress N acquisition, but its expression decreases under N-deficiency to promote N acquisition. On the contrary, PtTryp2 promotes phosphate uptake per se, and its expression increases under P-deficiency to further reinforce P acquisition. Furthermore, PtTryp2 knockout led to amplitude magnification of the nitrate and phosphate uptake ‘seesaw’, whereas PtTryp2 overexpression dampened it, linking PtTryp2 to stabilizing N:P stoichiometry. Our data demonstrate that PtTryp2 is a coordinate regulator of N:P stoichiometric homeostasis. The study opens a window for deciphering how phytoplankton adapt to nutrient-variable marine environments.

Suggested Citation

  • Yanchun You & Xueqiong Sun & Minglei Ma & Jiamin He & Ling Li & Felipe Wendt Porto & Senjie Lin, 2022. "Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31802-6
    DOI: 10.1038/s41467-022-31802-6
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    References listed on IDEAS

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    1. Quentin Carradec & Eric Pelletier & Corinne Da Silva & Adriana Alberti & Yoann Seeleuthner & Romain Blanc-Mathieu & Gipsi Lima-Mendez & Fabio Rocha & Leila Tirichine & Karine Labadie & Amos Kirilovsky, 2018. "A global ocean atlas of eukaryotic genes," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    2. Fayza Daboussi & Sophie Leduc & Alan Maréchal & Gwendoline Dubois & Valérie Guyot & Christophe Perez-Michaut & Alberto Amato & Angela Falciatore & Alexandre Juillerat & Marine Beurdeley & Daniel F. Vo, 2014. "Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology," Nature Communications, Nature, vol. 5(1), pages 1-7, September.
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    Cited by:

    1. Liqiang Yang & Xiaotong He & Shaoguo Ru & Yongyu Zhang, 2024. "Herbicide leakage into seawater impacts primary productivity and zooplankton globally," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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