IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-29840-1.html
   My bibliography  Save this article

The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei

Author

Listed:
  • Hoan X. Dinh

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Davinder Singh

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Diana Gomez de la Cruz

    (The Sainsbury Laboratory, University of East Anglia, Norwich Research Park)

  • Goetz Hensel

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK))

  • Jochen Kumlehn

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK))

  • Martin Mascher

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
    German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig)

  • Nils Stein

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
    Center of integrated Breeding Research (CiBreed), Department of Crop Sciences, Georg-August-University)

  • Dragan Perovic

    (Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance)

  • Michael Ayliffe

    (Commonwealth Scientific and Industrial Research Organisation)

  • Matthew J. Moscou

    (The Sainsbury Laboratory, University of East Anglia, Norwich Research Park)

  • Robert F. Park

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Mohammad Pourkheirandish

    (The University of Melbourne, Faculty of Veterinary and Agriculture)

Abstract

Leaf rust, caused by Puccinia hordei, is an economically significant disease of barley, but only a few major resistance genes to P. hordei (Rph) have been cloned. In this study, gene Rph3 was isolated by positional cloning and confirmed by mutational analysis and transgenic complementation. The Rph3 gene, which originated from wild barley and was first introgressed into cultivated Egyptian germplasm, encodes a unique predicted transmembrane resistance protein that differs from all known plant disease resistance proteins at the amino acid sequence level. Genetic profiles of diverse accessions indicated limited genetic diversity in Rph3 in domesticated germplasm, and higher diversity in wild barley from the Eastern Mediterranean region. The Rph3 gene was expressed only in interactions with Rph3-avirulent P. hordei isolates, a phenomenon also observed for transcription activator-like effector-dependent genes known as executors conferring resistance to Xanthomonas spp. Like known transmembrane executors such as Bs3 and Xa7, heterologous expression of Rph3 in N. benthamiana induced a cell death response. The isolation of Rph3 highlights convergent evolutionary processes in diverse plant-pathogen interaction systems, where similar defence mechanisms evolved independently in monocots and dicots.

Suggested Citation

  • Hoan X. Dinh & Davinder Singh & Diana Gomez de la Cruz & Goetz Hensel & Jochen Kumlehn & Martin Mascher & Nils Stein & Dragan Perovic & Michael Ayliffe & Matthew J. Moscou & Robert F. Park & Mohammad , 2022. "The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29840-1
    DOI: 10.1038/s41467-022-29840-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-29840-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-29840-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Alexei J Drummond & Simon Y W Ho & Matthew J Phillips & Andrew Rambaut, 2006. "Relaxed Phylogenetics and Dating with Confidence," PLOS Biology, Public Library of Science, vol. 4(5), pages 1-1, March.
    2. Murukarthick Jayakodi & Sudharsan Padmarasu & Georg Haberer & Venkata Suresh Bonthala & Heidrun Gundlach & Cécile Monat & Thomas Lux & Nadia Kamal & Daniel Lang & Axel Himmelbach & Jennifer Ens & Xiao, 2020. "The barley pan-genome reveals the hidden legacy of mutation breeding," Nature, Nature, vol. 588(7837), pages 284-289, December.
    3. Marscha Hirschi & Zachary Lee Johnson & Seok-Yong Lee, 2017. "Visualizing multistep elevator-like transitions of a nucleoside transporter," Nature, Nature, vol. 545(7652), pages 66-70, May.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Miaomiao Li & Huaizhi Zhang & Huixin Xiao & Keyu Zhu & Wenqi Shi & Dong Zhang & Yong Wang & Lijun Yang & Qiuhong Wu & Jingzhong Xie & Yongxing Chen & Dan Qiu & Guanghao Guo & Ping Lu & Beibei Li & Lei, 2024. "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Chunhong Chen & Matthias Jost & Megan A. Outram & Dorian Friendship & Jian Chen & Aihua Wang & Sambasivam Periyannan & Jan Bartoš & Kateřina Holušová & Jaroslav Doležel & Peng Zhang & Dhara Bhatt & Da, 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Joachim Schmidt & Lars Opgenoorth & Steffen Höll & Ralf Bastrop, 2012. "Into the Himalayan Exile: The Phylogeography of the Ground Beetle Ethira clade Supports the Tibetan Origin of Forest-Dwelling Himalayan Species Groups," PLOS ONE, Public Library of Science, vol. 7(9), pages 1-15, September.
    2. Iliana Bista & Jonathan M. D. Wood & Thomas Desvignes & Shane A. McCarthy & Michael Matschiner & Zemin Ning & Alan Tracey & James Torrance & Ying Sims & William Chow & Michelle Smith & Karen Oliver & , 2023. "Genomics of cold adaptations in the Antarctic notothenioid fish radiation," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Haitao Shang & Daniel H. Rothman & Gregory P. Fournier, 2022. "Oxidative metabolisms catalyzed Earth’s oxygenation," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    4. Mekala Sundaram & Janna R Willoughby & Nathanael I Lichti & Michael A Steele & Robert K Swihart, 2015. "Segregating the Effects of Seed Traits and Common Ancestry of Hardwood Trees on Eastern Gray Squirrel Foraging Decisions," PLOS ONE, Public Library of Science, vol. 10(6), pages 1-16, June.
    5. Jonathon D. Gass & Nichola J. Hill & Lambodhar Damodaran & Elena N. Naumova & Felicia B. Nutter & Jonathan A. Runstadler, 2023. "Ecogeographic Drivers of the Spatial Spread of Highly Pathogenic Avian Influenza Outbreaks in Europe and the United States, 2016–Early 2022," IJERPH, MDPI, vol. 20(11), pages 1-17, June.
    6. Xiaohua Yao & Leping Su & Youhua Yao & Likun An & Yixiong Bai & Xin Li & Kunlun Wu, 2022. "Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 58(1), pages 1-9.
    7. Nico Neureiter & Peter Ranacher & Nour Efrat-Kowalsky & Gereon A. Kaiping & Robert Weibel & Paul Widmer & Remco R. Bouckaert, 2022. "Detecting contact in language trees: a Bayesian phylogenetic model with horizontal transfer," Palgrave Communications, Palgrave Macmillan, vol. 9(1), pages 1-14, December.
    8. Michael D Nowak & Andrew B Smith & Carl Simpson & Derrick J Zwickl, 2013. "A Simple Method for Estimating Informative Node Age Priors for the Fossil Calibration of Molecular Divergence Time Analyses," PLOS ONE, Public Library of Science, vol. 8(6), pages 1-13, June.
    9. Bethany L Dearlove & Simon D W Frost, 2015. "Measuring Asymmetry in Time-Stamped Phylogenies," PLOS Computational Biology, Public Library of Science, vol. 11(7), pages 1-16, July.
    10. Idrissa Nonmon Sanogo & Claire Guinat & Simon Dellicour & Mohamed Adama Diakité & Mamadou Niang & Ousmane A Koita & Christelle Camus & Mariette F. Ducatez & Mariette Ducatez, 2024. "Genetic insights of H9N2 avian influenza viruses circulating in Mali and phylogeographic patterns in Northern and Western Africa," Post-Print hal-04498485, HAL.
    11. Matheus Pontes-Nogueira & Marcio Martins & Laura R V Alencar & Ricardo J Sawaya, 2021. "The role of vicariance and dispersal on the temporal range dynamics of forest vipers in the Neotropical region," PLOS ONE, Public Library of Science, vol. 16(9), pages 1-18, September.
    12. Sylvain Aubry, 2023. "Genebanking plant genetic resources in the postgenomic era," Agriculture and Human Values, Springer;The Agriculture, Food, & Human Values Society (AFHVS), vol. 40(3), pages 961-971, September.
    13. Cristian R Altaba, 2009. "Universal Artifacts Affect the Branching of Phylogenetic Trees, Not Universal Scaling Laws," PLOS ONE, Public Library of Science, vol. 4(2), pages 1-13, February.
    14. Rebecca L. Kordas & Samraat Pawar & Dimitrios-Georgios Kontopoulos & Guy Woodward & Eoin J. O’Gorman, 2022. "Metabolic plasticity can amplify ecosystem responses to global warming," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    15. Lokman Galal & Frédéric Ariey & Meriadeg Ar Gouilh & Marie-Laure Dardé & Azra Hamidović & Franck Letourneur & Franck Prugnolle & Aurélien Mercier, 2022. "A unique Toxoplasma gondii haplotype accompanied the global expansion of cats," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    16. Matthew Hall & Mark Woolhouse & Andrew Rambaut, 2015. "Epidemic Reconstruction in a Phylogenetics Framework: Transmission Trees as Partitions of the Node Set," PLOS Computational Biology, Public Library of Science, vol. 11(12), pages 1-36, December.
    17. Chunhong Chen & Matthias Jost & Megan A. Outram & Dorian Friendship & Jian Chen & Aihua Wang & Sambasivam Periyannan & Jan Bartoš & Kateřina Holušová & Jaroslav Doležel & Peng Zhang & Dhara Bhatt & Da, 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    18. Joanne S. Boden & Juntao Zhong & Rika E. Anderson & Eva E. Stüeken, 2024. "Timing the evolution of phosphorus-cycling enzymes through geological time using phylogenomics," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    19. Dinesh Aggarwal & Ben Warne & Aminu S. Jahun & William L. Hamilton & Thomas Fieldman & Louis Plessis & Verity Hill & Beth Blane & Emmeline Watkins & Elizabeth Wright & Grant Hall & Catherine Ludden & , 2022. "Genomic epidemiology of SARS-CoV-2 in a UK university identifies dynamics of transmission," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    20. Nan Song & Ai-Ping Liang, 2013. "A Preliminary Molecular Phylogeny of Planthoppers (Hemiptera: Fulgoroidea) Based on Nuclear and Mitochondrial DNA Sequences," PLOS ONE, Public Library of Science, vol. 8(3), pages 1-11, March.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29840-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.