IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjg/v58y2022i1id34-2021-cjgpb.html
   My bibliography  Save this article

Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley

Author

Listed:
  • Xiaohua Yao

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Leping Su

    (Qinghai University, Xining, P.R. China
    Yan'an Institute of Agricultural Sciences, Yan'an, P.R. China)

  • Youhua Yao

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Likun An

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Yixiong Bai

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Xin Li

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

  • Kunlun Wu

    (Qinghai University, Xining, P.R. China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, P.R. China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, P.R. China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, P.R. China)

Abstract

2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of the purple-grained Tibetan hulless barley variety Nerumuzha and the white-grained variety Kunlun 10. The HvnHID gene includes the 981 bp open reading frame and encodes a protein of 327 amino acids. It has a typical Abhydrolase_3 domain (78-306) and belongs to the carboxylesterase (CXE) family of the Abhydrolase_3 (α/β hydrolase) superfamily. There are eight nucleotide differences in the HvnHID coding sequence and two amino acid differences (one in the Abhydrolase_3 domain) between Nerumuzha and Kunlun 10. The HvnHID of hulless barley has the closest relationship with the HID in Hordeum vulgare, and the most distant relationship in Panicum hallii. At the early-mid stage of the seed colour development, the HvnHID expression levels in the purple and black seeds were significantly higher than in the white and blue ones (P < 0.01). During the seed colour development of purple-grained hulless barley, the expression of the key genes (HvnF3'H, HvnDRF, HvnANT1, and HvnGT) in the anthocyanidin biosynthetic pathway increased significantly, while the HvnHID expression decreased significantly (P < 0.01). Thus, it is likely that HvnHID negatively regulates the anthocyanidin biosynthesis. This result provides an important basis for further study of the biological functions of HvnHID in the anthocyanidin biosynthetic pathway.

Suggested Citation

  • Xiaohua Yao & Leping Su & Youhua Yao & Likun An & Yixiong Bai & Xin Li & Kunlun Wu, 2022. "Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 58(1), pages 1-9.
    • Handle: RePEc:caa:jnlcjg:v:58:y:2022:i:1:id:34-2021-cjgpb
    DOI: 10.17221/34/2021-CJGPB
    as

    Download full text from publisher

    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/34/2021-CJGPB.html
    Download Restriction: free of charge
    File URL: http://cjgpb.agriculturejournals.cz/doi/10.17221/34/2021-CJGPB.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/34/2021-CJGPB?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Murukarthick Jayakodi & Sudharsan Padmarasu & Georg Haberer & Venkata Suresh Bonthala & Heidrun Gundlach & Cécile Monat & Thomas Lux & Nadia Kamal & Daniel Lang & Axel Himmelbach & Jennifer Ens & Xiao, 2020. "The barley pan-genome reveals the hidden legacy of mutation breeding," Nature, Nature, vol. 588(7837), pages 284-289, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Linsan Liu & Sarah B. Jose & Chiara Campoli & Micha M. Bayer & Miguel A. Sánchez-Diaz & Trisha McAllister & Yichun Zhou & Mhmoud Eskan & Linda Milne & Miriam Schreiber & Thomas Batstone & Ian D. Bull , 2022. "Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Ting Wang & Shiyao Duan & Chen Xu & Yi Wang & Xinzhong Zhang & Xuefeng Xu & Liyang Chen & Zhenhai Han & Ting Wu, 2023. "Pan-genome analysis of 13 Malus accessions reveals structural and sequence variations associated with fruit traits," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Hoan X. Dinh & Davinder Singh & Diana Gomez de la Cruz & Goetz Hensel & Jochen Kumlehn & Martin Mascher & Nils Stein & Dragan Perovic & Michael Ayliffe & Matthew J. Moscou & Robert F. Park & Mohammad , 2022. "The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Sylvain Aubry, 2023. "Genebanking plant genetic resources in the postgenomic era," Agriculture and Human Values, Springer;The Agriculture, Food, & Human Values Society (AFHVS), vol. 40(3), pages 961-971, September.
    5. Carmen Escudero-Martinez & Max Coulter & Rodrigo Alegria Terrazas & Alexandre Foito & Rumana Kapadia & Laura Pietrangelo & Mauro Maver & Rajiv Sharma & Alessio Aprile & Jenny Morris & Pete E. Hedley &, 2022. "Identifying plant genes shaping microbiota composition in the barley rhizosphere," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    6. Chunhong Chen & Matthias Jost & Megan A. Outram & Dorian Friendship & Jian Chen & Aihua Wang & Sambasivam Periyannan & Jan Bartoš & Kateřina Holušová & Jaroslav Doležel & Peng Zhang & Dhara Bhatt & Da, 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjg:v:58:y:2022:i:1:id:34-2021-cjgpb. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.