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High-throughput production of functional prototissues capable of producing NO for vasodilation

Author

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  • Xiangxiang Zhang

    (State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology)

  • Chao Li

    (State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology)

  • Fukai Liu

    (Animal Laboratory Center, The First Affiliated Hospital of Harbin Medical University)

  • Wei Mu

    (State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology)

  • Yongshuo Ren

    (State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology)

  • Boyu Yang

    (State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology)

  • Xiaojun Han

    (State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology)

Abstract

Bottom-up synthesis of prototissues helps us to understand the internal cellular communications in the natural tissues and their functions, as well as to improve or repair the damaged tissues. The existed prototissues are rarely used to improve the function of living tissues. We demonstrate a methodology to produce spatially programmable prototissues based on the magneto-Archimedes effect in a high-throughput manner. More than 2000 prototissues are produced once within 2 h. Two-component and three-component spatial coded prototissues are fabricated by varying the addition giant unilamellar vesicles order/number, and the magnetic field distributions. Two-step and three-step signal communications in the prototissues are realized using cascade enzyme reactions. More importantly, the two-component prototissues capable of producing nitric oxide cause vasodilation of rat blood vessels in the presence of glucose and hydroxyurea. The tension force decreases 2.59 g, meanwhile the blood vessel relaxation is of 31.2%. Our works pave the path to fabricate complicated programmable prototissues, and hold great potential in the biomedical field.

Suggested Citation

  • Xiangxiang Zhang & Chao Li & Fukai Liu & Wei Mu & Yongshuo Ren & Boyu Yang & Xiaojun Han, 2022. "High-throughput production of functional prototissues capable of producing NO for vasodilation," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29571-3
    DOI: 10.1038/s41467-022-29571-3
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    References listed on IDEAS

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    1. Qingchuan Li & Shubin Li & Xiangxiang Zhang & Weili Xu & Xiaojun Han, 2020. "Programmed magnetic manipulation of vesicles into spatially coded prototissue architectures arrays," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    2. Guido Bolognesi & Mark S. Friddin & Ali Salehi-Reyhani & Nathan E. Barlow & Nicholas J. Brooks & Oscar Ces & Yuval Elani, 2018. "Sculpting and fusing biomimetic vesicle networks using optical tweezers," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    3. Alessandro Alcinesio & Oliver J. Meacock & Rebecca G. Allan & Carina Monico & Vanessa Restrepo Schild & Idil Cazimoglu & Matthew T. Cornall & Ravinash Krishna Kumar & Hagan Bayley, 2020. "Controlled packing and single-droplet resolution of 3D-printed functional synthetic tissues," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    4. Ahmet F. Demirörs & Pramod P. Pillai & Bartlomiej Kowalczyk & Bartosz A. Grzybowski, 2013. "Colloidal assembly directed by virtual magnetic moulds," Nature, Nature, vol. 503(7474), pages 99-103, November.
    5. Yuval Elani & Robert V. Law & Oscar Ces, 2014. "Vesicle-based artificial cells as chemical microreactors with spatially segregated reaction pathways," Nature Communications, Nature, vol. 5(1), pages 1-5, December.
    6. Liangfei Tian & Nicolas Martin & Philip G. Bassindale & Avinash J. Patil & Mei Li & Adrian Barnes & Bruce W. Drinkwater & Stephen Mann, 2016. "Spontaneous assembly of chemically encoded two-dimensional coacervate droplet arrays by acoustic wave patterning," Nature Communications, Nature, vol. 7(1), pages 1-10, December.
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    Cited by:

    1. Shubin Li & Yingming Zhao & Shuqi Wu & Xiangxiang Zhang & Boyu Yang & Liangfei Tian & Xiaojun Han, 2023. "Regulation of species metabolism in synthetic community systems by environmental pH oscillations," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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