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Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue

Author

Listed:
  • Haofeng Wang

    (Tianjin University
    Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
    Shanghai Clinical Research and Trial Center)

  • Yunjie Xiao

    (Tianjin University
    Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
    Shanghai Clinical Research and Trial Center
    Institute of Life Sciences, Chongqing Medical University)

  • Xia Chen

    (Tianjin University)

  • Mengwen Zhang

    (Yale University
    Yale University)

  • Guangxin Sun

    (Yale University)

  • Feng Wang

    (Tianjin University)

  • Lin Wang

    (Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
    Shanghai Clinical Research and Trial Center)

  • Hanxiao Zhang

    (Tianjin University)

  • Xiaoyu Zhang

    (Tianjin University
    Nankai University
    Tianjin International Joint Academy of Biotechnology and Medicine)

  • Xin Yang

    (Institute of Life Sciences, Chongqing Medical University)

  • Wenling Li

    (Tianjin University)

  • Yi Wei

    (Tianjin University)

  • Deqiang Yao

    (Shanghai Jiao Tong University)

  • Bing Zhang

    (Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University)

  • Jun Li

    (Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University)

  • Wen Cui

    (Tianjin University
    Institute of Life Sciences, Chongqing Medical University)

  • Fenghua Wang

    (Tianjin University)

  • Cheng Chen

    (Tianjin University)

  • Wei Shen

    (Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
    Shanghai Clinical Research and Trial Center)

  • Dan Su

    (Sichuan University and Collaborative Innovation Center for Biotherapy)

  • Fang Bai

    (Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
    Shanghai Clinical Research and Trial Center)

  • Jinhai Huang

    (Tianjin University)

  • Sheng Ye

    (Tianjin University)

  • Lei Zhang

    (Tianjin University)

  • Xiaoyun Ji

    (Nanjing University)

  • Wei Wang

    (Institute of Life Sciences, Chongqing Medical University)

  • Zefang Wang

    (Tianjin University
    Tianjin International Joint Academy of Biotechnology and Medicine)

  • Mark Hochstrasser

    (Yale University)

  • Haitao Yang

    (Tianjin University
    Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
    Shanghai Clinical Research and Trial Center
    Tianjin International Joint Academy of Biotechnology and Medicine)

Abstract

Cytoplasmic incompatibility (CI) results when Wolbachia bacteria-infected male insects mate with uninfected females, leading to embryonic lethality. “Rescue” of viability occurs if the female harbors the same Wolbachia strain. CI is caused by linked pairs of Wolbachia genes called CI factors (CifA and CifB). The co-evolution of CifA-CifB pairs may account in part for the incompatibility patterns documented in insects infected with different Wolbachia strains, but the molecular mechanisms remain elusive. Here, we use X-ray crystallography and AlphaFold to analyze the CI factors from Wolbachia strain wMel called CidAwMel and CidBwMel. Substituting CidAwMel interface residues with those from CidAwPip (from strain wPip) enables the mutant protein to bind CidBwPip and rescue CidBwPip-induced yeast growth defects, supporting the importance of CifA-CifB interaction in CI rescue. Sequence divergence in CidAwPip and CidBwPip proteins affects their pairwise interactions, which may help explain the complex incompatibility patterns of mosquitoes infected with different wPip strains.

Suggested Citation

  • Haofeng Wang & Yunjie Xiao & Xia Chen & Mengwen Zhang & Guangxin Sun & Feng Wang & Lin Wang & Hanxiao Zhang & Xiaoyu Zhang & Xin Yang & Wenling Li & Yi Wei & Deqiang Yao & Bing Zhang & Jun Li & Wen Cu, 2022. "Crystal Structures of Wolbachia CidA and CidB Reveal Determinants of Bacteria-induced Cytoplasmic Incompatibility and Rescue," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29273-w
    DOI: 10.1038/s41467-022-29273-w
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    References listed on IDEAS

    as
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    Cited by:

    1. Cameron J. McNamara & Thomas H. Ant & Tim Harvey-Samuel & Helen White-Cooper & Julien Martinez & Luke Alphey & Steven P. Sinkins, 2024. "Transgenic expression of cif genes from Wolbachia strain wAlbB recapitulates cytoplasmic incompatibility in Aedes aegypti," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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