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Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds

Author

Listed:
  • Mitchell Clough

    (Boston University)

  • Ichun Anderson Chen

    (Boston University
    Boston University)

  • Seong-Wook Park

    (Boston University
    Boston University)

  • Allison M. Ahrens

    (Boston University)

  • Jeffrey N. Stirman

    (University of North Carolina School of Medicine)

  • Spencer L. Smith

    (University of California Santa Barbara)

  • Jerry L. Chen

    (Boston University
    Boston University
    Boston University)

Abstract

Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

Suggested Citation

  • Mitchell Clough & Ichun Anderson Chen & Seong-Wook Park & Allison M. Ahrens & Jeffrey N. Stirman & Spencer L. Smith & Jerry L. Chen, 2021. "Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds," Nature Communications, Nature, vol. 12(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26737-3
    DOI: 10.1038/s41467-021-26737-3
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    References listed on IDEAS

    as
    1. Nathan G Clack & Daniel H O'Connor & Daniel Huber & Leopoldo Petreanu & Andrew Hires & Simon Peron & Karel Svoboda & Eugene W Myers, 2012. "Automated Tracking of Whiskers in Videos of Head Fixed Rodents," PLOS Computational Biology, Public Library of Science, vol. 8(7), pages 1-8, July.
    2. Johannes Friedrich & Pengcheng Zhou & Liam Paninski, 2017. "Fast online deconvolution of calcium imaging data," PLOS Computational Biology, Public Library of Science, vol. 13(3), pages 1-26, March.
    3. Jerry L. Chen & Stefano Carta & Joana Soldado-Magraner & Bernard L. Schneider & Fritjof Helmchen, 2013. "Behaviour-dependent recruitment of long-range projection neurons in somatosensory cortex," Nature, Nature, vol. 499(7458), pages 336-340, July.
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    Cited by:

    1. Che-Hang Yu & Jeffrey N. Stirman & Yiyi Yu & Riichiro Hira & Spencer L. Smith, 2021. "Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry," Nature Communications, Nature, vol. 12(1), pages 1-8, December.

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