IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v16y2023i5p2330-d1083519.html
   My bibliography  Save this article

Enhancing Biomass-Exopolysaccharides Production of Lignosus rhinocerus in a High-Scale Stirred-Tank Bioreactor and Its Potential Lipid as Bioenergy

Author

Listed:
  • Siti Rokhiyah Ahmad Usuldin

    (Agro-Biotechnology Institute (ABI), National Institutes of Biotechnology Malaysia (NIBM), Serdang 43400, Malaysia
    Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia)

  • Zul Ilham

    (Bioresources and Bioprocessing Research Group, Institute of Biological Sciences, Faculty of Sciences, Universiti Malaya, Kuala Lumpur 50603, Malaysia
    Environmental Science and Management Program, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia)

  • Adi Ainurzaman Jamaludin

    (Environmental Science and Management Program, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia)

  • Rahayu Ahmad

    (Halal Action Laboratory, Kolej GENIUS Insan, Universiti Sains Islam Malaysia, Bandar Baru Nilai, Nilai 71800, Malaysia)

  • Wan Abd Al Qadr Imad Wan-Mohtar

    (Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia
    Bioresources and Bioprocessing Research Group, Institute of Biological Sciences, Faculty of Sciences, Universiti Malaya, Kuala Lumpur 50603, Malaysia)

Abstract

The depletion of fossil fuels and the emission of greenhouse gases have increased the demand for new and sustainable energy sources, leading to growing interest in using fast-growing filamentous fungi as a source of bioenergy. This study aimed to optimize the production of exopolysaccharides (EPS) and mycelial biomass (MB) from the native medicinal mushroom, Lignosus rhinocerus, through submerged liquid fermentation. Using response surface methodology (RSM), it was found that the glucose concentration and speed of agitation significantly influenced the production of MB and EPS ( p < 0.05), while the initial pH medium had an insignificant effect. The validated optimized parameters of 50.0 g/L glucose, initial pH 4.0, and 128 rpm for speed of agitation were tested in 500 mL shake flasks, 5 L, and 13 L stirred-tank (STR) bioreactors. The production of MB and EPS increased significantly by ~1.2-fold in the 5 L STR and further increased to ~1.7-fold (MB) and ~2.4-fold (EPS) in the 13 L STR bioreactor compared to the shake flask. The lipid content of MB was also determined, with a result of 2.07% w / w using the Soxhlet extraction method. To conclude, this study emphasizes the ability of L. rhinocerus as a new source of bioenergy through large-scale production, with optimized parameters serving as a reference for future research and practical applications.

Suggested Citation

  • Siti Rokhiyah Ahmad Usuldin & Zul Ilham & Adi Ainurzaman Jamaludin & Rahayu Ahmad & Wan Abd Al Qadr Imad Wan-Mohtar, 2023. "Enhancing Biomass-Exopolysaccharides Production of Lignosus rhinocerus in a High-Scale Stirred-Tank Bioreactor and Its Potential Lipid as Bioenergy," Energies, MDPI, vol. 16(5), pages 1-18, February.
    • Handle: RePEc:gam:jeners:v:16:y:2023:i:5:p:2330-:d:1083519
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/16/5/2330/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/16/5/2330/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Percy, A. Jemila & Edwin, M., 2023. "Studies on the performance and emission characteristics of a dual fuel VCR engine using producer gas as secondary fuel: An optimization approach using response surface methodology," Energy, Elsevier, vol. 263(PA).
    2. Jerry L. Holechek & Hatim M. E. Geli & Mohammed N. Sawalhah & Raul Valdez, 2022. "A Global Assessment: Can Renewable Energy Replace Fossil Fuels by 2050?," Sustainability, MDPI, vol. 14(8), pages 1-22, April.
    3. Chong, Daniel Jia Sheng & Chan, Yi Jing & Arumugasamy, Senthil Kumar & Yazdi, Sara Kazemi & Lim, Jun Wei, 2023. "Optimisation and performance evaluation of response surface methodology (RSM), artificial neural network (ANN) and adaptive neuro-fuzzy inference system (ANFIS) in the prediction of biogas production ," Energy, Elsevier, vol. 266(C).
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Wei Wang & Leonid Melnyk & Oleksandra Kubatko & Bohdan Kovalov & Luc Hens, 2023. "Economic and Technological Efficiency of Renewable Energy Technologies Implementation," Sustainability, MDPI, vol. 15(11), pages 1-19, May.
    2. Zhang, Chao & Zhao, Yangsheng & Feng, Zijun & Meng, Qiaorong & Wang, Lei & Lu, Yang, 2023. "Thermal maturity and chemical structure evolution of lump long-flame coal during superheated water vapor–based in situ pyrolysis," Energy, Elsevier, vol. 263(PC).
    3. Ahmed Hussain Elmetwaly & Ramy Adel Younis & Abdelazeem Abdallah Abdelsalam & Ahmed Ibrahim Omar & Mohamed Metwally Mahmoud & Faisal Alsaif & Adel El-Shahat & Mohamed Attya Saad, 2023. "Modeling, Simulation, and Experimental Validation of a Novel MPPT for Hybrid Renewable Sources Integrated with UPQC: An Application of Jellyfish Search Optimizer," Sustainability, MDPI, vol. 15(6), pages 1-30, March.
    4. Daiva Makutėnienė & Algirdas Justinas Staugaitis & Bernardas Vaznonis & Gunta Grīnberga-Zālīte, 2023. "The Relationship between Energy Consumption and Economic Growth in the Baltic Countries’ Agriculture: A Non-Linear Framework," Energies, MDPI, vol. 16(5), pages 1-22, February.
    5. Evgeny Chupakhin & Olga Babich & Stanislav Sukhikh & Svetlana Ivanova & Ekaterina Budenkova & Olga Kalashnikova & Alexander Prosekov & Olga Kriger & Vyacheslav Dolganyuk, 2022. "Bioengineering and Molecular Biology of Miscanthus," Energies, MDPI, vol. 15(14), pages 1-14, July.
    6. Simona Domazetovska & Vladimir Strezov & Risto V. Filkoski & Tao Kan, 2023. "Exploring the Potential of Biomass Pyrolysis for Renewable and Sustainable Energy Production: A Comparative Study of Corn Cob, Vine Rod, and Sunflower," Sustainability, MDPI, vol. 15(18), pages 1-14, September.
    7. Ćalasan, Martin & Abdel Aleem, Shady H.E. & Hasanien, Hany M. & Alaas, Zuhair M. & Ali, Ziad M., 2023. "An innovative approach for mathematical modeling and parameter estimation of PEM fuel cells based on iterative Lambert W function," Energy, Elsevier, vol. 264(C).
    8. Grzegorz Pełka & Marta Jach-Nocoń & Marcin Paprocki & Artur Jachimowski & Wojciech Luboń & Adam Nocoń & Mateusz Wygoda & Paweł Wyczesany & Przemysław Pachytel & Tomasz Mirowski, 2023. "Comparison of Emissions and Efficiency of Two Types of Burners When Burning Wood Pellets from Different Suppliers," Energies, MDPI, vol. 16(4), pages 1-18, February.
    9. Yang Ni & Bin Peng & Jiayao Wang & Farshad Golnary & Wei Li, 2023. "A Short Review on the Time-Domain Numerical Simulations for Structural Responses in Horizontal-Axis Offshore Wind Turbines," Sustainability, MDPI, vol. 15(24), pages 1-19, December.
    10. Evgeniya I. Lysakova & Andrey V. Minakov & Angelica D. Skorobogatova, 2023. "Effect of Nanoparticle and Carbon Nanotube Additives on Thermal Stability of Hydrocarbon-Based Drilling Fluids," Energies, MDPI, vol. 16(19), pages 1-20, September.
    11. Shammya Afroze & Amal Najeebah Shalihah Binti Sofri & Md Sumon Reza & Zhanar Baktybaevna Iskakova & Asset Kabyshev & Kairat A. Kuterbekov & Kenzhebatyr Z. Bekmyrza & Lidiya Taimuratova & Mohammad Raki, 2023. "Solar-Powered Water Electrolysis Using Hybrid Solid Oxide Electrolyzer Cell (SOEC) for Green Hydrogen—A Review," Energies, MDPI, vol. 16(23), pages 1-22, November.
    12. Lu Wang & Zhijun Jin & Xiaowei Huang & Runchao Liu & Yutong Su & Qian Zhang, 2024. "Hydrogen Adsorption in Porous Geological Materials: A Review," Sustainability, MDPI, vol. 16(5), pages 1-21, February.
    13. Rafael Ninno Muniz & Carlos Tavares da Costa Júnior & William Gouvêa Buratto & Ademir Nied & Gabriel Villarrubia González, 2023. "The Sustainability Concept: A Review Focusing on Energy," Sustainability, MDPI, vol. 15(19), pages 1-22, September.
    14. Igor Tatarewicz & Sławomir Skwierz & Michał Lewarski & Robert Jeszke & Maciej Pyrka & Monika Sekuła, 2023. "Mapping the Future of Green Hydrogen: Integrated Analysis of Poland and the EU’s Development Pathways to 2050," Energies, MDPI, vol. 16(17), pages 1-27, August.
    15. César Ramírez-Márquez & Thelma Posadas-Paredes & Alma Yunuen Raya-Tapia & José María Ponce-Ortega, 2024. "Natural Resource Optimization and Sustainability in Society 5.0: A Comprehensive Review," Resources, MDPI, vol. 13(2), pages 1-20, January.
    16. Youchan Kim & Kisung Lim & Hassan Salihi & Seongku Heo & Hyunchul Ju, 2023. "The Effects of Stack Configurations on the Thermal Management Capabilities of Solid Oxide Electrolysis Cells," Energies, MDPI, vol. 17(1), pages 1-20, December.
    17. Dimitrios Vamvakas & Panagiotis Michailidis & Christos Korkas & Elias Kosmatopoulos, 2023. "Review and Evaluation of Reinforcement Learning Frameworks on Smart Grid Applications," Energies, MDPI, vol. 16(14), pages 1-38, July.
    18. Mohammed S. Almuhayawi & Elhagag A. Hassan & Saad Almasaudi & Nidal Zabermawi & Esam I. Azhar & Azhar Najjar & Khalil Alkuwaity & Turki S. Abujamel & Turki Alamri & Steve Harakeh, 2023. "Biodiesel Production through Rhodotorula toruloides Lipids and Utilization of De-Oiled Biomass for Congo Red Removal," Sustainability, MDPI, vol. 15(18), pages 1-22, September.
    19. Tiago Bastos & Leonor C. Teixeira & João C. O. Matias & Leonel J. R. Nunes, 2023. "Agroforestry Biomass Recovery Supply Chain Management: A More Efficient Information Flow Model Based on a Web Platform," Logistics, MDPI, vol. 7(3), pages 1-15, August.
    20. Lorenzo Colleoni & Alessio Sindoni & Silvia Ravelli, 2023. "Comprehensive Thermodynamic Evaluation of the Natural Gas-Fired Allam Cycle at Full Load," Energies, MDPI, vol. 16(6), pages 1-19, March.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:16:y:2023:i:5:p:2330-:d:1083519. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.