IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v13y2023i10p1875-d1247534.html
   My bibliography  Save this article

Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of SPL5 in Rice

Author

Listed:
  • Ping Li

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
    These authors contributed equally to this work.)

  • Nana Xu

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
    These authors contributed equally to this work.)

  • Yang Shui

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
    Rice Research Institute, Southwest University of Science and Technology, Mianyang 621010, China)

  • Jie Zhang

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
    Rice Research Institute, Southwest University of Science and Technology, Mianyang 621010, China)

  • Wuzhong Yin

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
    Rice Research Institute, Southwest University of Science and Technology, Mianyang 621010, China)

  • Min Tian

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)

  • Faping Guo

    (Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China)

  • Dasong Bai

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)

  • Pan Qi

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)

  • Qingxiong Huang

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)

  • Biluo Li

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)

  • Yuanyuan Li

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China)

  • Yungao Hu

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
    Rice Research Institute, Southwest University of Science and Technology, Mianyang 621010, China)

  • Youlin Peng

    (School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
    Rice Research Institute, Southwest University of Science and Technology, Mianyang 621010, China)

Abstract

This study was conducted on the lesion-mimic mutant lm5 , which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant lm5 was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, lm5 had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of lm5 , according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of LOC_Os07g10390 ( OsLM5 ) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The OsLM5 gene was found to be a new allele of the SPL5 gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and OsLM5 was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant lm5 , and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The OsLM5 gene may have a significant impact on the regulation of apoptosis in rice cells.

Suggested Citation

  • Ping Li & Nana Xu & Yang Shui & Jie Zhang & Wuzhong Yin & Min Tian & Faping Guo & Dasong Bai & Pan Qi & Qingxiong Huang & Biluo Li & Yuanyuan Li & Yungao Hu & Youlin Peng, 2023. "Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of SPL5 in Rice," Agriculture, MDPI, vol. 13(10), pages 1-16, September.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:10:p:1875-:d:1247534
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/13/10/1875/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/13/10/1875/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Zhen-Ming Pei & Yoshiyuki Murata & Gregor Benning & Sébastien Thomine & Birgit Klüsener & Gethyn J. Allen & Erwin Grill & Julian I. Schroeder, 2000. "Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells," Nature, Nature, vol. 406(6797), pages 731-734, August.
    2. Masashi Yamada & Xinwei Han & Philip N. Benfey, 2020. "RGF1 controls root meristem size through ROS signalling," Nature, Nature, vol. 577(7788), pages 85-88, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Helena KYSELÁKOVÁ & Michaela SEDLÁŘOVÁ & Martin KUBALA & Vladimíra NOŽKOVÁ & Jana PITERKOVÁ & Lenka LUHOVÁ & Ondřej NOVÁK & Petr ILÍK, 2013. "Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 49(3), pages 105-119.
    2. Zhang You & Shiyuan Guo & Qiao Li & Yanjun Fang & Panpan Huang & Chuanfeng Ju & Cun Wang, 2023. "The CBL1/9-CIPK1 calcium sensor negatively regulates drought stress by phosphorylating the PYLs ABA receptor," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Amandeep Kaur & Madhu & Alok Sharma & Kashmir Singh & Santosh Kumar Upadhyay, 2023. "Exploration of Piezo Channels in Bread Wheat ( Triticum aestivum L.)," Agriculture, MDPI, vol. 13(4), pages 1-16, March.
    4. Gilad Gabay & Hanchao Wang & Junli Zhang & Jorge I. Moriconi & German F. Burguener & Leonardo D. Gualano & Tyson Howell & Adam Lukaszewski & Brian Staskawicz & Myeong-Je Cho & Jaclyn Tanaka & Tzion Fa, 2023. "Dosage differences in 12-OXOPHYTODIENOATE REDUCTASE genes modulate wheat root growth," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    5. Tao Zhang & Sarah E. Noll & Jesus T. Peng & Amman Klair & Abigail Tripka & Nathan Stutzman & Casey Cheng & Richard N. Zare & Alexandra J. Dickinson, 2023. "Chemical imaging reveals diverse functions of tricarboxylic acid metabolites in root growth and development," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    6. Wen Shi & Lingyan Wang & Lianmei Yao & Wei Hao & Chao Han & Min Fan & Wenfei Wang & Ming-Yi Bai, 2022. "Spatially patterned hydrogen peroxide orchestrates stomatal development in Arabidopsis," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:13:y:2023:i:10:p:1875-:d:1247534. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.