IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v12y2022i9p1366-d904555.html
   My bibliography  Save this article

Effects of Nitrogen Addition on Soil Microbial Functional Diversity and Extracellular Enzyme Activities in Greenhouse Cucumber Cultivation

Author

Listed:
  • Zhen Wang

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Shuang Wang

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Ting Bian

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Qiaobo Song

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Guorui Wu

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Muhammad Awais

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Yufeng Liu

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Hongdan Fu

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

  • Zhouping Sun

    (College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
    Key Laboratory of Protected Horticulture of the Education Ministry and Liaoning Province, Shenyang 110866, China
    National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology, Shenyang 110866, China
    Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866, China)

Abstract

Greenhouses, commonly used for vegetable production, are experiencing large nitrogen (N) inputs in North China, which leads to soil acidification, increases soil N availability, and affects microbial community structure and composition. However, it remains unclear how N enrichment influences soil microbial functional activities in this region. In this study, we conducted a two-year pot experiment in a greenhouse to evaluate the effects of four different rates of N addition (0, 334, 668, and 1002 kg N ha −1 year −1 ) on cucumber soil properties, extracellular enzyme activities, and community level physiological profiles (CLPP). We found that high-N addition (1002 kg N ha −1 ) caused a massive accumulation of inorganic nitrogen and soil acidification, which was not beneficial to soil microbial activities. The color development (AWCD) values for the metabolism of microbial carbon sources and the activities of soil extracellular enzymes also showed a significant decrease in high N(N3) treatment. Additionally, the activity of leucine aminopeptidase (LAP) and polyphenol oxidase (PPO) of N3 decreased by 36% and 50% compared to the N0 and could be a good predictor for microbial functional diversity and microbial biomass carbon (MBC). Structural equation modeling (SEM) confirmed that the reduction of microbial functional diversity is mainly coregulated by the decline of soil pH and the change of cucumber BGB (belowground biomass) resulting from soil C and N imbalance. Overall, excessive N-fertilizer amendment can be more dangerous to microbial community functional diversity, especially for carbohydrate utilization which adversely affects cucumber yield in current intensive management.

Suggested Citation

  • Zhen Wang & Shuang Wang & Ting Bian & Qiaobo Song & Guorui Wu & Muhammad Awais & Yufeng Liu & Hongdan Fu & Zhouping Sun, 2022. "Effects of Nitrogen Addition on Soil Microbial Functional Diversity and Extracellular Enzyme Activities in Greenhouse Cucumber Cultivation," Agriculture, MDPI, vol. 12(9), pages 1-15, September.
    • Handle: RePEc:gam:jagris:v:12:y:2022:i:9:p:1366-:d:904555
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/12/9/1366/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/12/9/1366/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Nicolas Gruber & James N. Galloway, 2008. "An Earth-system perspective of the global nitrogen cycle," Nature, Nature, vol. 451(7176), pages 293-296, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Shen Yuan & Shaobing Peng, 2017. "Exploring the Trends in Nitrogen Input and Nitrogen Use Efficiency for Agricultural Sustainability," Sustainability, MDPI, vol. 9(10), pages 1-15, October.
    2. Keikha, Mahdi & Darzi- Naftchali, Abdullah & Motevali, Ali & Valipour, Mohammad, 2023. "Effect of nitrogen management on the environmental and economic sustainability of wheat production in different climates," Agricultural Water Management, Elsevier, vol. 276(C).
    3. Auguères, Anne-Sophie & Loreau, Michel, 2016. "Biotic regulation of non-limiting nutrient pools and coupling of biogeochemical cycles," Ecological Modelling, Elsevier, vol. 334(C), pages 1-7.
    4. Xiaochen Lu & Binjie Li & Guangsheng Chen, 2023. "Responses of Soil CO 2 Emission and Tree Productivity to Nitrogen and Phosphorus Additions in a Nitrogen-Rich Subtropical Chinese Fir Plantation," Sustainability, MDPI, vol. 15(12), pages 1-15, June.
    5. Florian Rabitz & Alin Olteanu & Jurgita Jurkevičienė & Agnė Budžytė, 2021. "A topic network analysis of the system turn in the environmental sciences," Scientometrics, Springer;Akadémiai Kiadó, vol. 126(3), pages 2107-2140, March.
    6. Chengpeng Zhang & Yu Ye & Xiuqi Fang & Hansunbai Li & Xue Zheng, 2020. "Coincidence Analysis of the Cropland Distribution of Multi-Sets of Global Land Cover Products," IJERPH, MDPI, vol. 17(3), pages 1-17, January.
    7. Sangha, Laljeet & Shortridge, Julie & Frame, William, 2023. "The impact of nitrogen treatment and short-term weather forecast data in irrigation scheduling of corn and cotton on water and nutrient use efficiency in humid climates," Agricultural Water Management, Elsevier, vol. 283(C).
    8. Huang, Suo & Bartlett, Paul & Arain, M. Altaf, 2016. "An analysis of global terrestrial carbon, water and energy dynamics using the carbon–nitrogen coupled CLASS-CTEMN+ model," Ecological Modelling, Elsevier, vol. 336(C), pages 36-56.
    9. L.J. Li & D.H. Zeng & R. Mao & Z.Y. Yu, 2012. "Nitrogen and phosphorus resorption of Artemisia scoparia, Chenopodium acuminatum, Cannabis sativa, and Phragmites communis under nitrogen and phosphorus additions in a semiarid grassland, China," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 58(10), pages 446-451.
    10. Yusen Chen & Shihang Zhang & Yongdong Wang, 2022. "Distribution Characteristics and Drivers of Soil Carbon and Nitrogen in the Drylands of Central Asia," Land, MDPI, vol. 11(10), pages 1-12, October.
    11. Charles A. Taylor & Geoffrey Heal, 2021. "Fertilizer and Algal Blooms: A Satellite Approach to Assessing Water Quality," NBER Chapters, in: Risks in Agricultural Supply Chains, National Bureau of Economic Research, Inc.
    12. Chen, Minpeng & Sun, Fu & Shindo, Junko, 2016. "China’s agricultural nitrogen flows in 2011: Environmental assessment and management scenarios," Resources, Conservation & Recycling, Elsevier, vol. 111(C), pages 10-27.
    13. Rong Zhang & Chuan Li & Huilin Cui & Yanbo Wang & Shaoce Zhang & Pei Li & Yue Hou & Ying Guo & Guojin Liang & Zhaodong Huang & Chao Peng & Chunyi Zhi, 2023. "Electrochemical nitrate reduction in acid enables high-efficiency ammonia synthesis and high-voltage pollutes-based fuel cells," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    14. Smith, Nicola J & McDonald, Garry W & Patterson, Murray G, 2020. "Biogeochemical cycling in the anthropocene: Quantifying global environment-economy exchanges," Ecological Modelling, Elsevier, vol. 418(C).
    15. Gu, Baojing & Liu, Dong & Wu, Xu & Ge, Ying & Min, Yong & Jiang, Hong & Chang, Jie, 2011. "Utilization of waste nitrogen for biofuel production in China," Renewable and Sustainable Energy Reviews, Elsevier, vol. 15(9), pages 4910-4916.
    16. Zhen-Zhen Zheng & Li-Wei Zheng & Min Nina Xu & Ehui Tan & David A. Hutchins & Wenchao Deng & Yao Zhang & Dalin Shi & Minhan Dai & Shuh-Ji Kao, 2020. "Substrate regulation leads to differential responses of microbial ammonia-oxidizing communities to ocean warming," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    17. Barakat, Mohammad & Cheviron, Bruno & Angulo-Jaramillo, Rafael, 2016. "Influence of the irrigation technique and strategies on the nitrogen cycle and budget: A review," Agricultural Water Management, Elsevier, vol. 178(C), pages 225-238.
    18. Douglas, Niall Edward, 2008. "Modelling the Costs of Climate Change and its Costs of Mitigation: A Scientific Approach," MPRA Paper 13650, University Library of Munich, Germany.
    19. Stops, Marven W. & Sullivan, Pamela L. & Peltier, Edward & Young, Bryan & Brookfield, Andrea E., 2022. "Tracking the hydrologic response of agricultural tile outlet terraces to storm events," Agricultural Water Management, Elsevier, vol. 263(C).
    20. Jian Sha & Zeli Li & Dennis Swaney & Bongghi Hong & Wei Wang & Yuqiu Wang, 2014. "Application of a Bayesian Watershed Model Linking Multivariate Statistical Analysis to Support Watershed-Scale Nitrogen Management in China," Water Resources Management: An International Journal, Published for the European Water Resources Association (EWRA), Springer;European Water Resources Association (EWRA), vol. 28(11), pages 3681-3695, September.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:12:y:2022:i:9:p:1366-:d:904555. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.