IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v51y2006i5id5551-vetmed.html
   My bibliography  Save this article

Risk assessment of mycobacterial infections (human tuberculosis and avian mycobacteriosis) during anatomical dissection of cadavers

Author

Listed:
  • M. Bartos

    (Veterinary Research Institute, Brno, Czech Republic)

  • H. Pavlikova

    (Masaryk University, Brno, Czech Republic
    Veterinary and Pharmaceutical University, Brno, Czech Republic)

  • L. Dvorska

    (Veterinary Research Institute, Brno, Czech Republic)

  • R. Horvath

    (Genex CZ, Ltd., Brno, Czech Republic)

  • M. Dendis

    (Genex CZ, Ltd., Brno, Czech Republic)

  • P. Flodr

    (Palacky University, Olomouc, Czech Republic)

  • Z. Kolar

    (Palacky University, Olomouc, Czech Republic)

  • R.T. Weston

    (Veterinary Research Institute, Brno, Czech Republic
    LaTrobe University, Melbourne, Australia)

  • L. Pac

    (Masaryk University, Brno, Czech Republic)

  • L. Matlova

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

The aim of this work was to study the presence of mycobacteria in tissue samples from four cadavers fixed with formalin, and tissue samples from a recently deceased unpreserved individual, who had a history of human tuberculosis infection, undergoing a post mortem (cause of death not related to tuberculosis). All were examined for the presence of tuberculous lesions and the specific presence of Mycobacterium tuberculosis complex (MTC) and M. avium complex (MAC) members by microscopy, culture, and PCR analysis of four genomic elements (IS6110, mtp40, IS901, and IS1245). Microscopy examination after the Ziehl-Neelsen staining and culture examination for the presence of mycobacteria were negative in all 22 tissue samples from the four embalmed cadavers. PCR analysis of IS6110 and mtp40 was positive in tissue samples of tuberculous lesions from the lungs of two embalmed cadavers, and from intact kidney tissue of one of these cadavers. Microscopy and culture examinations of liver and spleen tissues from the unpreserved cadaver were positive for mycobacteria. PCR analysis, specific for M. avium subsp. avium, was positive in both tissue samples with, and without tuberculous lesions.

Suggested Citation

  • M. Bartos & H. Pavlikova & L. Dvorska & R. Horvath & M. Dendis & P. Flodr & Z. Kolar & R.T. Weston & L. Pac & L. Matlova & I. Pavlik, 2006. "Risk assessment of mycobacterial infections (human tuberculosis and avian mycobacteriosis) during anatomical dissection of cadavers," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 311-319.
    • Handle: RePEc:caa:jnlvet:v:51:y:2006:i:5:id:5551-vetmed
    DOI: 10.17221/5551-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5551-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/5551-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/5551-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. L. Dvorská & M. Bartoš & G. Martin & W. Erler & I. Pavlík, 2001. "Strategies for differentiation, identification and typing of medically important species of mycobacteria by molecular methods," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 46(11-12), pages 309-328.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. J.E. Shitaye & B. Getahun & T. Alemayehu & M. Skoric & F. Treml & P. Fictum & V. Vrbas & I. Pavlik, 2006. "A prevalence study of bovine tuberculosis by using abattoir meat inspection and tuberculin skin testing data, histopathological and IS6110 PCR examination of tissues with tuberculous lesions in cattle," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(11), pages 512-522.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. L. Matlova & L. Dvorska & J. Bartl & M. Bartos & W.Y. Ayele & M. Alexa & I. Pavlik, 2003. "Mycobacteria isolated from the environment of pig farms in the Czech Republic during the years 1996 to 2002," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(12), pages 343-357.
    2. P. Lescenko & L. Matlova & L. Dvorska & M. Bartos & O. Vavra & S. Navratil & L. Novotny & I. Pavlik, 2003. "Mycobacterial infection in aquarium fish," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(3), pages 71-78.
    3. I. Pavlik & L. Dvorska & M. Bartos & I. Parmova & I. Melicharek & A. Jesenska & M. Havelkova & M. Slosarek & I. Putova & G. Martin & W. Erler & K. Kremer & D. Van Soolingen, 2002. "Molecular epidemiology of bovine tuberculosis in the Czech Republic and Slovakia in the period 1965-2001 studied by spoligotyping," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(7), pages 181-194.
    4. I. Pavlik & F. Bures & P. Janovsky & P. Pecinka & M. Bartos & L. Dvorska & L. Matlova & K. Kremer & D. Van Soolingen, 2002. "The last outbreak of bovine tuberculosis in cattle in the Czech Republic in 1995 was caused by Mycobacterium bovis subspecies caprae," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(9), pages 251-263.
    5. M. Bartos & J. O. Falkinham III & I. Pavlik, 2004. "Mycobacterial catalases, peroxidases, and superoxide dismutases and their effects on virulence and isoniazid-susceptibility in mycobacteria - a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(5), pages 161-170.
    6. M. Skoric & E.J. Shitaye & R. Halouzka & P. Fictum & I. Trcka & M. Heroldova & E. Tkadlec & I. Pavlik, 2007. "Tuberculous and tuberculoid lesions in free living small terrestrial mammals and the risk of infection to humans and animals: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 52(4), pages 144-161.
    7. L. Novotny & L. Dvorska & A. Lorencova & V. Beran & I. Pavlik, 2004. "Fish: a potential source of bacterial pathogens for human beings," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(9), pages 343-358.
    8. J. Hosek & P. Svastova & M. Moravkova & I. Pavlik & M. Bartos, 2006. "Methods of mycobacterial DNA isolation from different biological material: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 180-192.
    9. I. Pavlik & L. Matlova & L. Dvorska & J. Bartl & L. Oktabcova & J. Docekal & I. Parmova, 2003. "Tuberculous lesions in pigs in the Czech Republic in the years 1990-1999: occurrence, causal factors and economic losses," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 48(5), pages 113-125.
    10. K. Hruska, 2004. "Research on paratuberculosis: Analysis of publications 1994-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(8), pages 271-282.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:51:y:2006:i:5:id:5551-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.