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Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae

Author

Listed:
  • I. Holko

    (State Veterinary Institute, Prague, Czech Republic)

  • J. Urbanova

    (State Veterinary Institute, Prague, Czech Republic)

  • Tholkova

    (State Veterinary Institute, Prague, Czech Republic)

  • V. Kmet

    (State Veterinary Institute, Prague, Czech Republic)

Abstract

The main goal of our work is the presentation and analysis of incidence of porcine respiratory disease complex (PRDC) regarding bacterial agents in the territory of northern districts of Slovakia. Mycoplasma hyopneumoniae and other secondary bacterial causative pathogens of PRDC comprised 75.2% of all cases (98) with clinical signs of respiratory infections that we examined in the course of one year. We present also one of possibilities to the solution of problematic detection of M. hyopneumoniae which is, like the whole rank of mycoplasmas, very difficult to cultivate. This problem was solved by using the PCR method with the direct isolation of M. hyopneumoniae from lungs tissue. In antibiotic sensitivity testing of Pasteurella multocida and Actinobacillus pleuropneumoniae resulted enrofloxacin as the most effective antibiotics in the therapy of PRDC regarding bacterial agents.in above mentioned territory.

Suggested Citation

  • I. Holko & J. Urbanova & Tholkova & V. Kmet, 2004. "Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(2), pages 35-41.
    • Handle: RePEc:caa:jnlvet:v:49:y:2004:i:2:id:5672-vetmed
    DOI: 10.17221/5672-VETMED
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    References listed on IDEAS

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    1. P. Šatrán & K. Nedbalcová, 2002. "Prevalence of serotypes, production of Apx toxins, and antibiotic resistance in strains of Actinobacillus pleuropneumoniae isolated in the Czech Republic," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 47(4), pages 92-98.
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    Cited by:

    1. K. Nedbalcova & P. Satran & Z. Jaglic & R. Ondriasova & Z. Kucerova, 2005. "Monitoring of antibiotic resistance in isolates of Actinobacillus pleuropneumoniaein the Czech Republic between 2001 and 2003," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(5), pages 181-185.

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    1. K. Nedbalcova & P. Satran & Z. Jaglic & R. Ondriasova & Z. Kucerova, 2005. "Monitoring of antibiotic resistance in isolates of Actinobacillus pleuropneumoniaein the Czech Republic between 2001 and 2003," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(5), pages 181-185.
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