IDEAS home Printed from https://ideas.repec.org/a/caa/jnlpps/v57y2021i3id180-2020-pps.html
   My bibliography  Save this article

Use of gene family analysis to discover argonaut (AGO) genes for increasing the resistance of Tibetan hull-less barley to leaf stripe disease

Author

Listed:
  • Xiaohua Yao

    (Qinghai University, Xining, China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, China)

  • Yue Wang

    (Qinghai University, Xining, China)

  • Youhua Yao

    (Qinghai University, Xining, China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, China)

  • Likun An

    (Qinghai University, Xining, China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, China)

  • Yixiong Bai

    (Qinghai University, Xining, China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, China)

  • Xin Li

    (Qinghai University, Xining, China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, China)

  • Kunlun Wu

    (Qinghai University, Xining, China
    Qinghai Academy of Agricultural and Forestry Sciences, Xining, China
    Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China
    Qinghai Subcenter of National Hulless Barley Improvement, Xining, China)

  • Youming Qiao

    (Qinghai University, Xining, China)

Abstract

Leaf stripe is a common, but major infectious disease of barley, severely affecting the yield and quality. However, only a few genes have been identified by conventional gene mapping. Gene family analysis has become a fast and efficient strategy for gene discovery. Studies demonstrated that Argonaute (AGO) proteins play an important role in plant disease resistance. Thus, we obtained nine HvAGO genes via mRNA sequencing before and after a Pyrenophora graminea infection of a disease-resistant variety "Kunlun 14" and a susceptible variety "Z1141". We analysed the physicochemical characteristics, gene structures, and motifs of the HvAGO gene sequences and found that these proteins were divided into four clusters by evolutionary distance. There was high consistency in the number of exons, size, and the number and type of motifs in the different clusters. Based on protein phylogenetics, they could be divided into three branches. A collinearity analysis of Tibetan hull-less barley and Arabidopsis thaliana, rice, and maize showed that four genes were collinear with respect to the other three species. The qRT-PCR showed the expression levels of HvAGO1, HvAGO2 and HvAGO4 were significantly increased after infection with Pyrenophora graminea. These three members of the AGO gene family are, thus, speculated to play an important role in barley leaf stripe resistance. The results provide reference for the application of HvAGO genes in the leaf stripe control and the exploration of disease resistance genes in other crops.

Suggested Citation

  • Xiaohua Yao & Yue Wang & Youhua Yao & Likun An & Yixiong Bai & Xin Li & Kunlun Wu & Youming Qiao, 2021. "Use of gene family analysis to discover argonaut (AGO) genes for increasing the resistance of Tibetan hull-less barley to leaf stripe disease," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 57(3), pages 226-239.
    • Handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:180-2020-pps
    DOI: 10.17221/180/2020-PPS
    as

    Download full text from publisher

    File URL: http://pps.agriculturejournals.cz/doi/10.17221/180/2020-PPS.html
    Download Restriction: free of charge
    File URL: http://pps.agriculturejournals.cz/doi/10.17221/180/2020-PPS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/180/2020-PPS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Yanli Wang & Stefan Juranek & Haitao Li & Gang Sheng & Thomas Tuschl & Dinshaw J. Patel, 2008. "Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex," Nature, Nature, vol. 456(7224), pages 921-926, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Chikako Ragan & Michael Zuker & Mark A Ragan, 2011. "Quantitative Prediction of miRNA-mRNA Interaction Based on Equilibrium Concentrations," PLOS Computational Biology, Public Library of Science, vol. 7(2), pages 1-11, February.
    2. Xiangkai Zhen & Xiaolong Xu & Le Ye & Song Xie & Zhijie Huang & Sheng Yang & Yanhui Wang & Jinyu Li & Feng Long & Songying Ouyang, 2024. "Structural basis of antiphage immunity generated by a prokaryotic Argonaute-associated SPARSA system," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Yonghua Wang & Yan Li & Zhi Ma & Wei Yang & Chunzhi Ai, 2010. "Mechanism of MicroRNA-Target Interaction: Molecular Dynamics Simulations and Thermodynamics Analysis," PLOS Computational Biology, Public Library of Science, vol. 6(7), pages 1-19, July.
    4. Sarah Willkomm & Leonhard Jakob & Kevin Kramm & Veronika Graus & Julia Neumeier & Gunter Meister & Dina Grohmann, 2022. "Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    5. Hanlun Jiang & Fu Kit Sheong & Lizhe Zhu & Xin Gao & Julie Bernauer & Xuhui Huang, 2015. "Markov State Models Reveal a Two-Step Mechanism of miRNA Loading into the Human Argonaute Protein: Selective Binding followed by Structural Re-arrangement," PLOS Computational Biology, Public Library of Science, vol. 11(7), pages 1-21, July.

    More about this item

    Keywords

    gene expression; HvAGO1; HvAGO2; HvAGO4; Pyrenophora graminea;
    All these keywords.

    JEL classification:

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlpps:v:57:y:2021:i:3:id:180-2020-pps. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.