IDEAS home Printed from https://ideas.repec.org/p/ags/cfcs90/259333.html
   My bibliography  Save this paper

HISTOCHEMICAL AND BIOCHEMICAL STUDIES ON TUBERIZING ROOTS IN SWEET POTATO (Ipomoea batatas L., CV 049)

Author

Listed:
  • Babwah, A.V.
  • Sirju-Charran, G.

Abstract

Histochemical studies on roots of sweet potato at different stages of development revealed the presence of guaiacol oxidase and 3,3-diaminobenzidine (DAB) oxidase activities in all cells except for xylem vessels. In roots at primary and secondary stages of development similar activities were observed with highest activities in the endodermal and companion cells. In primary roots high activities were also observed in the hypodermal cells. In roots showing secondary growth cells of the periderm, which developed from the hypodermis, as well as xylem tracheids continued to show high activities. Syringaldazine oxidase showed a lag phase with weak activity in the cortical cells and high activities in all cells undergoing lignification in roots at all stages of development. Biochemical assays of guaiacol oxidase indicated high activities in both the soluble cell free (CF) and the solubilized ionically wall bound (I) enzyme fractions with the I fraction consistently showing higher activities in all stages of tuberizing roots. Qualitative tests showed that syringaldazine oxidase activity was restricted to the CF fraction. Both cofactor dependent and independent indole acetic acid (IAA) oxidase were assayed with only the I fraction in all stages of tuberizing roots showing cofactor dependent IAA oxidase activity. The implications of these results are discussed in relation to the anatomical changes and physiological processes taking place in the developing tuberous root.

Suggested Citation

  • Babwah, A.V. & Sirju-Charran, G., 1990. "HISTOCHEMICAL AND BIOCHEMICAL STUDIES ON TUBERIZING ROOTS IN SWEET POTATO (Ipomoea batatas L., CV 049)," 26th Annual Meeting, July 29 to August 4, 1990, Mayaguez, Puerto Rico 259333, Caribbean Food Crops Society.
    • Handle: RePEc:ags:cfcs90:259333
    DOI: 10.22004/ag.econ.259333
    as

    Download full text from publisher

    File URL: https://ageconsearch.umn.edu/record/259333/files/26_14.pdf
    Download Restriction: no
    File URL: https://libkey.io/10.22004/ag.econ.259333?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:ags:cfcs90:259333. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: AgEcon Search (email available below). General contact details of provider: http://cfcs.eea.uprm.edu/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.